Detailed information of TA system TA080894

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4447937..4448769
• Replicon: chromosome
• Accession: NZ_CP023357
• Organism: Escherichia coli strain 317

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CNQ55_RS22595
• Protein ID: WP_047615650.1
• Coordinates: 4447937..4448311 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A4D2S730
• Locus tag: CNQ55_RS22600
• Protein ID: WP_042965901.1
• Coordinates: 4448401..4448769 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ55_RS22565	4443439..4443618	+	180	WP_001298063.1	hypothetical protein	-
CNQ55_RS22570	4444017..4446053	-	2037	WP_000417001.1	hypothetical protein	-
CNQ55_RS26715	4447006..4447158	-	153	WP_001280445.1	hypothetical protein	-
CNQ55_RS22585	4447243..4447440	-	198	WP_000839281.1	DUF957 domain-containing protein	-
CNQ55_RS22590	4447452..4447940	-	489	WP_000777547.1	hypothetical protein	-
CNQ55_RS22595	4447937..4448311	-	375	WP_047615650.1	TA system toxin CbtA family protein	Toxin
CNQ55_RS22600	4448401..4448769	-	369	WP_042965901.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CNQ55_RS22605	4448849..4449070	-	222	WP_000692350.1	DUF987 domain-containing protein	-
CNQ55_RS22610	4449157..4449633	-	477	WP_123005962.1	RadC family protein	-
CNQ55_RS22615	4449649..4450128	-	480	WP_096037620.1	antirestriction protein	-
CNQ55_RS22620	4450394..4451212	-	819	WP_001175153.1	DUF945 domain-containing protein	-
CNQ55_RS22625	4451302..4451535	-	234	WP_001278287.1	DUF905 family protein	-
CNQ55_RS22630	4451541..4452218	-	678	WP_001097301.1	hypothetical protein	-
CNQ55_RS22635	4452366..4453046	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4437356..4490243	52887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14158.10 Da
• Isoelectric Point: 7.3526

>T80894 WP_047615650.1 NZ_CP023357:c4448311-4447937 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T80894 NZ_CP023357:c4448311-4447937 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.49 Da
• Isoelectric Point: 6.9465

>AT80894 WP_042965901.1 NZ_CP023357:c4448769-4448401 [Escherichia coli]
VSDTLPGTTHPGDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT80894 NZ_CP023357:c4448769-4448401 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGGCGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A4D2S730