Detailed information of TA system TA080886

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3980335..3981170
• Replicon: chromosome
• Accession: NZ_CP023357
• Organism: Escherichia coli strain 317

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: CNQ55_RS20250
• Protein ID: WP_000854700.1
• Coordinates: 3980335..3980712 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: CNQ55_RS20255
• Protein ID: WP_001278232.1
• Coordinates: 3980802..3981170 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ55_RS20220	3975657..3976151	+	495	WP_001059463.1	MarR family transcriptional regulator	-
CNQ55_RS20225	3976586..3976924	-	339	WP_021552747.1	Arm DNA-binding domain-containing protein	-
CNQ55_RS20230	3977290..3978609	+	1320	WP_000144686.1	site-specific integrase	-
CNQ55_RS20235	3978702..3979556	-	855	WP_024174313.1	DUF4942 domain-containing protein	-
CNQ55_RS20240	3979641..3979838	-	198	WP_000839247.1	DUF957 domain-containing protein	-
CNQ55_RS20245	3979850..3980338	-	489	WP_000761726.1	hypothetical protein	-
CNQ55_RS20250	3980335..3980712	-	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
CNQ55_RS20255	3980802..3981170	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CNQ55_RS20260	3981333..3981554	-	222	WP_000692353.1	DUF987 domain-containing protein	-
CNQ55_RS20265	3981623..3982099	-	477	WP_001186199.1	RadC family protein	-
CNQ55_RS20270	3982115..3982600	-	486	WP_000213723.1	antirestriction protein	-
CNQ55_RS20275	3982692..3983510	-	819	WP_001234709.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat	3932674..4009070	76396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T80886 WP_000854700.1 NZ_CP023357:c3980712-3980335 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T80886 NZ_CP023357:c3980712-3980335 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT80886 WP_001278232.1 NZ_CP023357:c3981170-3980802 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT80886 NZ_CP023357:c3981170-3980802 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A1I2

Antitoxin

• AlphaFold DB: E3PJ72