Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1177737..1178391 Replicon chromosome
Accession NZ_CP023357
Organism Escherichia coli strain 317

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag CNQ55_RS05980 Protein ID WP_000244765.1
Coordinates 1177984..1178391 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CNQ55_RS05975 Protein ID WP_000354046.1
Coordinates 1177737..1178003 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ55_RS05955 1173825..1175258 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
CNQ55_RS05960 1175303..1175614 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
CNQ55_RS05965 1175778..1176437 + 660 WP_000250274.1 hemolysin III family protein -
CNQ55_RS05970 1176514..1177494 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
CNQ55_RS05975 1177737..1178003 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CNQ55_RS05980 1177984..1178391 + 408 WP_000244765.1 protein YgfX Toxin
CNQ55_RS05985 1178431..1178952 - 522 WP_001055874.1 flavodoxin FldB -
CNQ55_RS05990 1179064..1179960 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CNQ55_RS05995 1179985..1180695 + 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CNQ55_RS06000 1180701..1182434 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T80870 WP_000244765.1 NZ_CP023357:1177984-1178391 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T80870 NZ_CP023357:1177984-1178391 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT80870 WP_000354046.1 NZ_CP023357:1177737-1178003 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT80870 NZ_CP023357:1177737-1178003 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References