Detailed information of TA system TA080869

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1002042..1002877
• Replicon: chromosome
• Accession: NZ_CP023357
• Organism: Escherichia coli strain 317

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CNQ55_RS05055
• Protein ID: WP_096037512.1
• Coordinates: 1002042..1002419 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CNQ55_RS05060
• Protein ID: WP_096037513.1
• Coordinates: 1002509..1002877 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ55_RS05025	997176..998318	-	1143	WP_033556765.1	capsule biosynthesis protein	-
CNQ55_RS05030	998390..999373	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
CNQ55_RS05035	1000169..1000339	-	171	Protein_928	IS110 family transposase	-
CNQ55_RS05040	1000682..1001251	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
CNQ55_RS05045	1001348..1001545	-	198	WP_000839281.1	DUF957 domain-containing protein	-
CNQ55_RS05050	1001557..1002045	-	489	WP_001519085.1	hypothetical protein	-
CNQ55_RS05055	1002042..1002419	-	378	WP_096037512.1	TA system toxin CbtA family protein	Toxin
CNQ55_RS05060	1002509..1002877	-	369	WP_096037513.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CNQ55_RS05065	1003040..1003261	-	222	WP_000692329.1	DUF987 domain-containing protein	-
CNQ55_RS05070	1003324..1003800	-	477	WP_001186726.1	RadC family protein	-
CNQ55_RS05075	1003816..1004301	-	486	WP_000849582.1	antirestriction protein	-
CNQ55_RS05080	1004356..1005174	-	819	WP_001234620.1	DUF945 domain-containing protein	-
CNQ55_RS05090	1005274..1005507	-	234	WP_001119729.1	DUF905 family protein	-
CNQ55_RS05095	1005586..1006041	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1000169..1000273	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14002.94 Da
• Isoelectric Point: 7.3522

>T80869 WP_096037512.1 NZ_CP023357:c1002419-1002042 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T80869 NZ_CP023357:c1002419-1002042 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCGGGTATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTTCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACAGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13544.44 Da
• Isoelectric Point: 6.9558

>AT80869 WP_096037513.1 NZ_CP023357:c1002877-1002509 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT80869 NZ_CP023357:c1002877-1002509 [Escherichia coli]
GTGTCAGACGCACTCTCTGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGAGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA