Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 2188..2735 Replicon plasmid p62
Accession NZ_CP023354
Organism Escherichia coli strain 746

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag CNQ56_RS25875 Protein ID WP_000969996.1
Coordinates 2454..2735 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E0WH52
Locus tag CNQ56_RS25870 Protein ID WP_000079932.1
Coordinates 2188..2457 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ56_RS25850 348..422 + 75 WP_001365705.1 RepA leader peptide Tap -
CNQ56_RS25855 415..1272 + 858 WP_000130647.1 incFII family plasmid replication initiator RepA -
CNQ56_RS25870 2188..2457 + 270 WP_000079932.1 hypothetical protein Antitoxin
CNQ56_RS25875 2454..2735 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CNQ56_RS25880 2999..4186 + 1188 WP_000905831.1 hypothetical protein -
CNQ56_RS25885 4275..5186 + 912 WP_024244186.1 toll/interleukin-1 receptor domain-containing protein -
CNQ56_RS25890 5328..5723 - 396 WP_000445930.1 plasmid partitioning/stability family protein -
CNQ56_RS25895 5723..6682 - 960 WP_000921958.1 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..62312 62312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T80853 WP_000969996.1 NZ_CP023354:2454-2735 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T80853 NZ_CP023354:2454-2735 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10268.58 Da        Isoelectric Point: 7.2055

>AT80853 WP_000079932.1 NZ_CP023354:2188-2457 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVSDK

Download         Length: 270 bp

>AT80853 NZ_CP023354:2188-2457 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTTCCGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5U8RN22

References