Detailed information of TA system TA080848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4369331..4370151
• Replicon: chromosome
• Accession: NZ_CP023353
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: CNQ56_RS22100
• Protein ID: WP_001054376.1
• Coordinates: 4369331..4369588 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: CNQ56_RS22105
• Protein ID: WP_000215866.1
• Coordinates: 4369600..4370151 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS22070	4364617..4365723	+	1107	WP_001044124.1	N-acetylneuraminate epimerase	-
CNQ56_RS22075	4365788..4366768	+	981	WP_001765419.1	sialate O-acetylesterase	-
CNQ56_RS22085	4367351..4368577	-	1227	Protein_4038	helicase YjhR	-
CNQ56_RS22090	4368655..4368954	+	300	WP_024186563.1	GNAT family N-acetyltransferase	-
CNQ56_RS22100	4369331..4369588	+	258	WP_001054376.1	hypothetical protein	Toxin
CNQ56_RS22105	4369600..4370151	+	552	WP_000215866.1	N-acetyltransferase	Antitoxin
CNQ56_RS22110	4370203..4370949	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
CNQ56_RS22115	4371076..4371336	+	261	WP_000084876.1	hypothetical protein	-
CNQ56_RS28060	4371374..4371490	+	117	Protein_4044	VOC family protein	-
CNQ56_RS22125	4371735..4372856	+	1122	WP_000010835.1	M42 family metallopeptidase	-
CNQ56_RS22130	4372853..4373131	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
CNQ56_RS22135	4373143..4374456	+	1314	WP_000460846.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	4358359..4378371	20012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T80848 WP_001054376.1 NZ_CP023353:4369331-4369588 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T80848 NZ_CP023353:4369331-4369588 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20493.64 Da
• Isoelectric Point: 6.8758

>AT80848 WP_000215866.1 NZ_CP023353:4369600-4370151 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKETDLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT80848 NZ_CP023353:4369600-4370151 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAACCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: S1PA82