Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4322473..4323049 Replicon chromosome
Accession NZ_CP023353
Organism Escherichia coli strain 746

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag CNQ56_RS21855 Protein ID WP_001131965.1
Coordinates 4322762..4323049 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag CNQ56_RS21850 Protein ID WP_019842999.1
Coordinates 4322473..4322775 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ56_RS21825 4318167..4318679 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
CNQ56_RS21835 4319069..4321219 + 2151 WP_001439331.1 pyruvate/proton symporter BtsT -
CNQ56_RS21840 4321269..4321472 + 204 WP_000467859.1 YbdD/YjiX family protein -
CNQ56_RS21845 4321483..4322439 + 957 WP_001295525.1 GTPase -
CNQ56_RS21850 4322473..4322775 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
CNQ56_RS21855 4322762..4323049 - 288 WP_001131965.1 BrnT family toxin Toxin
CNQ56_RS21860 4323246..4324412 + 1167 WP_000800825.1 restriction endonuclease -
CNQ56_RS21865 4324476..4326095 + 1620 WP_001029743.1 type I restriction-modification system subunit M -
CNQ56_RS21870 4326085..4327509 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T80847 WP_001131965.1 NZ_CP023353:c4323049-4322762 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T80847 NZ_CP023353:c4323049-4322762 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT80847 WP_019842999.1 NZ_CP023353:c4322775-4322473 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT80847 NZ_CP023353:c4322775-4322473 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCAATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References