Detailed information of TA system TA080846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4201257..4201681
• Replicon: chromosome
• Accession: NZ_CP023353
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CNQ56_RS28055
• Protein ID: WP_001586653.1
• Coordinates: 4201257..4201445 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: CNQ56_RS21240
• Protein ID: WP_000125566.1
• Coordinates: 4201448..4201681 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS21210	4196819..4198105	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
CNQ56_RS21215	4198105..4199094	+	990	WP_000241280.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CNQ56_RS21220	4199091..4199912	+	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CNQ56_RS21225	4199915..4200292	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CNQ56_RS21230	4200299..4201147	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CNQ56_RS28050	4201144..4201260	-	117	WP_001389634.1	CcdB family protein	-
CNQ56_RS28055	4201257..4201445	-	189	WP_001586653.1	CcdB family protein	Toxin
CNQ56_RS21240	4201448..4201681	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CNQ56_RS21245	4201767..4202246	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
CNQ56_RS21250	4202438..4204300	-	1863	WP_000377117.1	glutathione-regulated potassium-efflux system protein KefC	-
CNQ56_RS21255	4204293..4204823	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CNQ56_RS21260	4204931..4206262	-	1332	WP_001183198.1	MFS transporter	-
CNQ56_RS21265	4206321..4206608	-	288	WP_001596509.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7029.22 Da
• Isoelectric Point: 8.7938

>T80846 WP_001586653.1 NZ_CP023353:c4201445-4201257 [Escherichia coli]
MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T80846 NZ_CP023353:c4201445-4201257 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGTAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCAGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT80846 WP_000125566.1 NZ_CP023353:c4201681-4201448 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT80846 NZ_CP023353:c4201681-4201448 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9