Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3751229..3751847 Replicon chromosome
Accession NZ_CP023353
Organism Escherichia coli strain 746

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CNQ56_RS19035 Protein ID WP_001291435.1
Coordinates 3751629..3751847 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CNQ56_RS19030 Protein ID WP_000344800.1
Coordinates 3751229..3751603 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ56_RS19020 3746318..3747511 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CNQ56_RS19025 3747534..3750683 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
CNQ56_RS19030 3751229..3751603 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CNQ56_RS19035 3751629..3751847 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CNQ56_RS19040 3752019..3752570 + 552 WP_000102564.1 maltose O-acetyltransferase -
CNQ56_RS19045 3752686..3753156 + 471 WP_000136192.1 YlaC family protein -
CNQ56_RS19050 3753320..3754870 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CNQ56_RS19055 3754912..3755265 - 354 WP_001550741.1 DUF1428 family protein -
CNQ56_RS19065 3755644..3755955 + 312 WP_000409911.1 MGMT family protein -
CNQ56_RS19070 3755986..3756558 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T80843 WP_001291435.1 NZ_CP023353:3751629-3751847 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T80843 NZ_CP023353:3751629-3751847 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT80843 WP_000344800.1 NZ_CP023353:3751229-3751603 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT80843 NZ_CP023353:3751229-3751603 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References