Detailed information of TA system TA080842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3348856..3349561
• Replicon: chromosome
• Accession: NZ_CP023353
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CNQ56_RS17110
• Protein ID: WP_000539521.1
• Coordinates: 3348856..3349242 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CNQ56_RS17115
• Protein ID: WP_001280945.1
• Coordinates: 3349232..3349561 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS17090	3344860..3345486	+	627	WP_001595548.1	glutathione S-transferase GstB	-
CNQ56_RS17095	3345483..3346598	-	1116	WP_001595547.1	aldose sugar dehydrogenase YliI	-
CNQ56_RS17100	3346709..3347092	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CNQ56_RS17105	3347305..3348630	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CNQ56_RS17110	3348856..3349242	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNQ56_RS17115	3349232..3349561	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CNQ56_RS17120	3349631..3350959	-	1329	WP_000086872.1	GGDEF domain-containing protein	-
CNQ56_RS17125	3350967..3353315	-	2349	WP_001595546.1	EAL domain-containing protein	-
CNQ56_RS17130	3353493..3354404	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T80842 WP_000539521.1 NZ_CP023353:3348856-3349242 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T80842 NZ_CP023353:3348856-3349242 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT80842 WP_001280945.1 NZ_CP023353:3349232-3349561 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT80842 NZ_CP023353:3349232-3349561 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6