Detailed information of TA system TA080841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3094149..3094933
• Replicon: chromosome
• Accession: NZ_CP023353
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: CNQ56_RS15800
• Protein ID: WP_001595626.1
• Coordinates: 3094439..3094933 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: CNQ56_RS15795
• Protein ID: WP_001110447.1
• Coordinates: 3094149..3094442 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS15780	3089299..3090258	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
CNQ56_RS15790	3090831..3094016	+	3186	WP_001595627.1	ribonuclease E	-
CNQ56_RS15795	3094149..3094442	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
CNQ56_RS15800	3094439..3094933	+	495	WP_001595626.1	GNAT family N-acetyltransferase	Toxin
CNQ56_RS15805	3095028..3095981	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
CNQ56_RS15810	3095993..3097636	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
CNQ56_RS15815	3097702..3098643	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
CNQ56_RS15820	3098643..3099740	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18065.97 Da
• Isoelectric Point: 8.2617

>T80841 WP_001595626.1 NZ_CP023353:3094439-3094933 [Escherichia coli]
MIQAPEPLIARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T80841 NZ_CP023353:3094439-3094933 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATTGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT80841 WP_001110447.1 NZ_CP023353:3094149-3094442 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT80841 NZ_CP023353:3094149-3094442 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: L4JCW6