Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1072712..1073366 Replicon chromosome
Accession NZ_CP023353
Organism Escherichia coli strain 746

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CNQ56_RS05605 Protein ID WP_000244781.1
Coordinates 1072959..1073366 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CNQ56_RS05600 Protein ID WP_000354046.1
Coordinates 1072712..1072978 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ56_RS05580 1068800..1070233 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
CNQ56_RS05585 1070278..1070589 + 312 WP_001525592.1 N(4)-acetylcytidine aminohydrolase -
CNQ56_RS05590 1070753..1071412 + 660 WP_000250275.1 hemolysin III family protein -
CNQ56_RS05595 1071489..1072469 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
CNQ56_RS05600 1072712..1072978 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CNQ56_RS05605 1072959..1073366 + 408 WP_000244781.1 protein YgfX Toxin
CNQ56_RS05610 1073406..1073927 - 522 WP_001055874.1 flavodoxin FldB -
CNQ56_RS05615 1074039..1074935 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CNQ56_RS05620 1074960..1075670 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CNQ56_RS05625 1075676..1077409 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T80827 WP_000244781.1 NZ_CP023353:1072959-1073366 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T80827 NZ_CP023353:1072959-1073366 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT80827 WP_000354046.1 NZ_CP023353:1072712-1072978 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT80827 NZ_CP023353:1072712-1072978 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References