Detailed information of TA system TA080825

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 882890..883685
• Replicon: chromosome
• Accession: NZ_CP023353
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A658LKE6
• Locus tag: CNQ56_RS04475
• Protein ID: WP_001596171.1
• Coordinates: 882890..883264 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CNQ56_RS04480
• Protein ID: WP_001596170.1
• Coordinates: 883311..883685 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS04445	878972..879943	-	972	WP_157739648.1	KpsF/GutQ family sugar-phosphate isomerase	-
CNQ56_RS04450	880754..880924	-	171	Protein_813	IS110 family transposase	-
CNQ56_RS04455	881266..882108	-	843	Protein_814	DUF4942 domain-containing protein	-
CNQ56_RS04465	882193..882390	-	198	WP_001596173.1	DUF957 domain-containing protein	-
CNQ56_RS04470	882402..882893	-	492	WP_001596172.1	hypothetical protein	-
CNQ56_RS04475	882890..883264	-	375	WP_001596171.1	TA system toxin CbtA family protein	Toxin
CNQ56_RS04480	883311..883685	-	375	WP_001596170.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CNQ56_RS04485	883848..884069	-	222	WP_000692323.1	DUF987 domain-containing protein	-
CNQ56_RS04490	884132..884608	-	477	WP_001186726.1	RadC family protein	-
CNQ56_RS04495	884624..885076	-	453	WP_096035156.1	antirestriction protein	-
CNQ56_RS27915	886308..886370	-	63	Protein_823	antirestriction protein	-
CNQ56_RS04505	886462..886944	-	483	Protein_824	DUF932 domain-containing protein	-
CNQ56_RS04510	887068..887301	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	880754..886241	5487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14161.14 Da
• Isoelectric Point: 6.7041

>T80825 WP_001596171.1 NZ_CP023353:c883264-882890 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLARLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYELVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T80825 NZ_CP023353:c883264-882890 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGTTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCGC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACTCCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGAACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13813.62 Da
• Isoelectric Point: 6.7387

>AT80825 WP_001596170.1 NZ_CP023353:c883685-883311 [Escherichia coli]
VSDTLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT80825 NZ_CP023353:c883685-883311 [Escherichia coli]
GTGTCAGACACACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGAGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A658LKE6