Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 34379..35022 Replicon plasmid unnamed3
Accession NZ_CP023352
Organism Escherichia coli strain ETEC-2264

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag CJU63_RS27305 Protein ID WP_001044768.1
Coordinates 34379..34795 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID E9XUD3
Locus tag CJU63_RS27310 Protein ID WP_001261279.1
Coordinates 34792..35022 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU63_RS27275 30257..31446 + 1190 WP_164724826.1 IS3 family transposase -
CJU63_RS28020 31650..32216 + 567 WP_000587875.1 hypothetical protein -
CJU63_RS27285 32284..32493 - 210 Protein_28 transposase domain-containing protein -
CJU63_RS27290 32537..32680 + 144 Protein_29 transposase -
CJU63_RS27295 33051..33656 - 606 WP_000515888.1 hypothetical protein -
CJU63_RS27305 34379..34795 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
CJU63_RS27310 34792..35022 - 231 WP_001261279.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CJU63_RS27315 35717..36001 + 285 WP_001331078.1 hypothetical protein -
CJU63_RS27320 35994..36683 + 690 WP_000796232.1 hypothetical protein -
CJU63_RS27325 36680..37468 + 789 WP_000016497.1 site-specific integrase -
CJU63_RS27330 37649..38293 + 645 WP_001144036.1 ParA family protein -
CJU63_RS27335 38380..38688 + 309 WP_000030199.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - etpB / etpA 1..45777 45777


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T80823 WP_001044768.1 NZ_CP023352:c34795-34379 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T80823 NZ_CP023352:c34795-34379 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8761.90 Da        Isoelectric Point: 4.6310

>AT80823 WP_001261279.1 NZ_CP023352:c35022-34792 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPVRPAWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT80823 NZ_CP023352:c35022-34792 [Escherichia coli]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGCCCGGCCTGGGGCTCGTTCGCGCAGCTCG
AGAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGCCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A3U0EQM4

References