Detailed information of TA system TA080818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 41209..41768
• Replicon: plasmid unnamed1
• Accession: NZ_CP023350
• Organism: Escherichia coli strain ETEC-2264

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: CJU63_RS26080
• Protein ID: WP_000421259.1
• Coordinates: 41209..41484 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: CJU63_RS26085
• Protein ID: WP_001178087.1
• Coordinates: 41484..41768 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU63_RS26065	36738..37364	-	627	WP_000620425.1	ATP-binding cassette domain-containing protein	-
CJU63_RS27725	37345..38130	-	786	WP_000775193.1	hypothetical protein	-
CJU63_RS27730	38087..39292	-	1206	WP_000673444.1	TolC family protein	-
CJU63_RS26075	39289..40425	-	1137	WP_000465104.1	hypothetical protein	-
CJU63_RS26080	41209..41484	-	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CJU63_RS26085	41484..41768	-	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
CJU63_RS26105	42675..43706	-	1032	WP_003964539.1	replication initiation protein	-
CJU63_RS26120	44678..44941	+	264	WP_024167927.1	ash family protein	-
CJU63_RS26125	44859..45146	+	288	WP_000074855.1	conjugal transfer protein TraA	-
CJU63_RS26135	45588..46121	+	534	WP_001712498.1	transcription termination factor nusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltA / eltB / csvA	1..112045	112045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T80818 WP_000421259.1 NZ_CP023350:c41484-41209 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T80818 NZ_CP023350:c41484-41209 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACAAGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT80818 WP_001178087.1 NZ_CP023350:c41768-41484 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT80818 NZ_CP023350:c41768-41484 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAAATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGTCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625