Detailed information of TA system TA080817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4851153..4852836
• Replicon: chromosome
• Accession: NZ_CP023349
• Organism: Escherichia coli strain ETEC-2264

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: CJU63_RS25200
• Protein ID: WP_001385928.1
• Coordinates: 4851153..4852070 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: CJU63_RS25205
• Protein ID: WP_000157137.1
• Coordinates: 4852063..4852836 (-)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU63_RS25180	4847763..4848782	-	1020	WP_001349988.1	NADPH-dependent aldehyde reductase Ahr	-
CJU63_RS25190	4849263..4849973	+	711	WP_001219054.1	integrase arm-type DNA-binding domain-containing protein	-
CJU63_RS25195	4849977..4850639	+	663	WP_000357928.1	hypothetical protein	-
CJU63_RS27970	4850692..4850859	-	168	WP_001356018.1	hypothetical protein	-
CJU63_RS25200	4851153..4852070	-	918	WP_001385928.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CJU63_RS25205	4852063..4852836	-	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
CJU63_RS25210	4852920..4853240	+	321	Protein_4619	IS3 family transposase	-
CJU63_RS25215	4853416..4854765	-	1350	WP_000766444.1	phospholipase	-
CJU63_RS25220	4854884..4856968	-	2085	WP_001193075.1	protease Lon-related BREX system protein BrxL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34944.07 Da
• Isoelectric Point: 4.9206

>T80817 WP_001385928.1 NZ_CP023349:c4852070-4851153 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQISEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T80817 NZ_CP023349:c4852070-4851153 [Escherichia coli]
ATGGATAAAACCTCCCCCTACTACCGCCAGGTCGCTCTGTTGGTGCGAGCATTACCCTGGGTCGCTAACGAGTCCTGTTT
TGCGCTGAAAGGCGGAACGGCGATCAACTTATTCATCCATGATTTTCCCCGATTGTCCGTTGATATCGATCTGGCCTGGA
TACCGCTGGAAAGCAGAGAGGTCGCGCTGCCCAACGTGCGGGAGGCGCTCACCCGTATCGCCGCAGAACTGAAAGCGCAA
ACCGGGATCCGTGCAGTGCTGCAAACCCATAATCCCGATGAGATGCGCATCATTGTCACCACTGACGATGCGCAAATTAA
AATTGAAGTCTCGCCAGTTGCCCGGGGGACACTATATCCTCCGCAAGAACGCGATATCGTCGGAGCCGTGGAGGAAGAAT
TTGGCTTTGCCGCGCTCCCGGTGGTCTCACTGCCCGATTTATACGGTGGCAAGCTATGTGCTGCGTTAGATCGCCAGCAC
CCCAGAGATTTTTACGACGTCAAAATGCTCCTTGATACACAAGGAATTGATCGCTCGATATTCAATGGTTTTATCGTGTA
CTTGCTCAGCCATAACCGCCCATTATCCGAAGTGCTTAACCCACGTTGGAAGGATATTACTGAACCCTTTCAAAATGAAT
TCAGCGGTATGACTTTTGAGGAAGTATCACTGGAAGAGCTAAAAGAGGTCCCGGAAAGAATGCTCAGCACGTTGAAAACG
CATTTCACTCAACAGGATTTCGACTTCCTGATGTCATTTAAACGCGGCGAGCCAGACTGGAATTTAGCGCCAGAGCCCCA
CATTCAGAATTTGCCAGCCGTTCAATGGAAGCTGCGCAATATTGGACAGATATCGGAGGAAAAGCGCGCGCAGTCACTGG
AGAAACTAGAAAAGGTCATGACAGAATGGCTACGTTAG

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT80817 WP_000157137.1 NZ_CP023349:c4852836-4852063 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT80817 NZ_CP023349:c4852836-4852063 [Escherichia coli]
ATGGCATCAAAATTAAACTGGCTGTTGCAAAACACCTCTCCGGGAAGCCTGGTGTTACAATCGTGGCTGACCCGGAACAA
CATATCACCTTCTCTGGCTTTTAAATACGCGCAAAATGGCTGGTTAATAAAACTGCGGGCTGGCGTTTATGCGCGCGCAG
GACGAGAACCAGACTGGAGTGATGCGCTATGGTGCCTACAAAACCAACTTGAAGCGCCGGTGCATCTGGCCGGACTGAGT
AGTCTCTCCTGGCAGGGCCGTTCCCATTACCTACAACTTAAGCAGAACCAATGCTGGCTTAGCGTTGAAAACAAAGCGAT
ATTGCCAAAATGGTTTAAAGAATTTCCCGCCGTTGAGTGGTTGATGGTATCGGCCCTGAAGCTCCCTGACCTGGATGAAA
AATACCGAACTGAGCTTGAGGTTAAAGGGAAAAGAATTACCGCCAGCACGCCAGAACTGGCTGCCTATGAGCTGTTAAGC
GCAGTCCCTCAGGAAATCTCATTTGAGCACGCTGCCGAGCTTTTTCAGGGACTAGTCAATCTCAACCCACGTAAAGTGGA
GAAGCTGCTTACACTCAGTCAGTCCGTACAAACCAAGCGCCTGTATCTGTTTTTTGCCAGTTTCTATGAACATGCATGGT
TCAAACGCATAGATACCAGCAAAATCAACCTGGGATCGGGCAACCGACAAATCGTTGTTAATGGCAAGCTGAACACGCAA
TACCAGATAACTGTGCCCGAAAATTTTACTAAGAAGGGAATAAGCCATGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2