Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3939115..3939746 Replicon chromosome
Accession NZ_CP023349
Organism Escherichia coli strain ETEC-2264

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag CJU63_RS20660 Protein ID WP_001259385.1
Coordinates 3939471..3939746 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag CJU63_RS20655 Protein ID WP_000593554.1
Coordinates 3939115..3939474 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU63_RS20625 3934227..3934700 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
CJU63_RS20630 3934697..3934978 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
CJU63_RS20635 3935055..3936413 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
CJU63_RS20640 3936406..3937914 + 1509 WP_001298718.1 carbohydrate kinase -
CJU63_RS20645 3937904..3938173 + 270 WP_000933060.1 HPr family phosphocarrier protein -
CJU63_RS20650 3938205..3939065 + 861 WP_001131783.1 class II aldolase -
CJU63_RS20655 3939115..3939474 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CJU63_RS20660 3939471..3939746 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
CJU63_RS20665 3939819..3940943 - 1125 WP_001314210.1 ABC transporter permease -
CJU63_RS20670 3940943..3943678 - 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
CJU63_RS20675 3943675..3944742 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T80814 WP_001259385.1 NZ_CP023349:c3939746-3939471 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T80814 NZ_CP023349:c3939746-3939471 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT80814 WP_000593554.1 NZ_CP023349:c3939474-3939115 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT80814 NZ_CP023349:c3939474-3939115 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References