Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3258521..3259175 Replicon chromosome
Accession NZ_CP023349
Organism Escherichia coli strain ETEC-2264

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag CJU63_RS16920 Protein ID WP_000244772.1
Coordinates 3258521..3258928 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CJU63_RS16925 Protein ID WP_000354046.1
Coordinates 3258909..3259175 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU63_RS16900 3254478..3256211 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
CJU63_RS16905 3256217..3256927 - 711 WP_095892512.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CJU63_RS16910 3256952..3257848 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CJU63_RS16915 3257960..3258481 + 522 WP_001055874.1 flavodoxin FldB -
CJU63_RS16920 3258521..3258928 - 408 WP_000244772.1 protein YgfX Toxin
CJU63_RS16925 3258909..3259175 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CJU63_RS16930 3259418..3260398 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CJU63_RS16935 3260475..3261134 - 660 WP_000250274.1 hemolysin III family protein -
CJU63_RS16940 3261298..3261609 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CJU63_RS16945 3261654..3263087 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
CJU63_RS16950 3263144..3263887 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T80811 WP_000244772.1 NZ_CP023349:c3258928-3258521 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T80811 NZ_CP023349:c3258928-3258521 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT80811 WP_000354046.1 NZ_CP023349:c3259175-3258909 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT80811 NZ_CP023349:c3259175-3258909 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References