Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2770112..2770737 Replicon chromosome
Accession NZ_CP023349
Organism Escherichia coli strain ETEC-2264

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CJU63_RS14425 Protein ID WP_000911329.1
Coordinates 2770112..2770510 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CJU63_RS14430 Protein ID WP_000450524.1
Coordinates 2770510..2770737 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU63_RS14405 2766019..2766219 + 201 WP_000383836.1 YpfN family protein -
CJU63_RS14410 2766300..2766998 - 699 WP_000679812.1 esterase -
CJU63_RS14415 2767072..2769087 - 2016 WP_001388162.1 tRNA cytosine(34) acetyltransferase TmcA -
CJU63_RS14420 2769102..2769965 - 864 WP_001267498.1 neutral zinc metallopeptidase -
CJU63_RS14425 2770112..2770510 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CJU63_RS14430 2770510..2770737 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CJU63_RS14435 2770892..2771605 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CJU63_RS14440 2771818..2772852 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CJU63_RS14445 2772869..2773747 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CJU63_RS14450 2773893..2774465 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CJU63_RS14455 2774465..2774935 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T80809 WP_000911329.1 NZ_CP023349:c2770510-2770112 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T80809 NZ_CP023349:c2770510-2770112 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT80809 WP_000450524.1 NZ_CP023349:c2770737-2770510 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT80809 NZ_CP023349:c2770737-2770510 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References