Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 504661..505279 Replicon chromosome
Accession NZ_CP023349
Organism Escherichia coli strain ETEC-2264

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CJU63_RS02490 Protein ID WP_001291435.1
Coordinates 504661..504879 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CJU63_RS02495 Protein ID WP_000344800.1
Coordinates 504905..505279 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU63_RS02455 499951..500523 + 573 WP_000779831.1 YbaY family lipoprotein -
CJU63_RS02460 500554..500865 - 312 WP_000409911.1 MGMT family protein -
CJU63_RS02470 501244..501597 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CJU63_RS02475 501639..503189 - 1551 WP_001387361.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CJU63_RS02480 503353..503823 - 471 WP_000136192.1 YlaC family protein -
CJU63_RS02485 503939..504490 - 552 WP_000102578.1 maltose O-acetyltransferase -
CJU63_RS02490 504661..504879 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CJU63_RS02495 504905..505279 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CJU63_RS02500 505825..508974 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CJU63_RS02505 508997..510190 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T80798 WP_001291435.1 NZ_CP023349:c504879-504661 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T80798 NZ_CP023349:c504879-504661 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT80798 WP_000344800.1 NZ_CP023349:c505279-504905 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT80798 NZ_CP023349:c505279-504905 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References