Detailed information of TA system TA080795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 97534..98177
• Replicon: plasmid unnamed1
• Accession: NZ_CP023347
• Organism: Escherichia coli strain ETEC-2265

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q2VNX7
• Locus tag: CJU64_RS27210
• Protein ID: WP_001044769.1
• Coordinates: 97761..98177 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: T2HRV4
• Locus tag: CJU64_RS27205
• Protein ID: WP_001261281.1
• Coordinates: 97534..97764 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU64_RS27165	92734..93081	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CJU64_RS28780	93078..93458	-	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CJU64_RS27175	93815..94621	-	807	WP_000099345.1	site-specific integrase	-
CJU64_RS27180	94622..94927	-	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	-
CJU64_RS27185	94929..95147	-	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	-
CJU64_RS27195	95702..96391	+	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CJU64_RS27200	96425..97042	-	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CJU64_RS27205	97534..97764	+	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CJU64_RS27210	97761..98177	+	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	Toxin
CJU64_RS27215	98353..99471	+	1119	Protein_123	IS3 family transposase	-
CJU64_RS27220	99935..101179	+	1245	WP_000115575.1	hypothetical protein	-
CJU64_RS27225	101198..101347	+	150	WP_001399277.1	ATP-binding protein	-
CJU64_RS28785	101741..101968	-	228	WP_001731352.1	bacterial lipid A biosynthesis acyltransferase family protein	-
CJU64_RS27235	102220..102846	-	627	WP_000593827.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	csfA / cssA	1..142368	142368
	-	inside	IScluster/Tn	-	-	79439..99380	19941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15183.63 Da
• Isoelectric Point: 7.7805

>T80795 WP_001044769.1 NZ_CP023347:97761-98177 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T80795 NZ_CP023347:97761-98177 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8769.89 Da
• Isoelectric Point: 4.3775

>AT80795 WP_001261281.1 NZ_CP023347:97534-97764 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPIRPTWGSFLECEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT80795 NZ_CP023347:97534-97764 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCATCCGGCCAACCTGGGGATCATTCCTAGAGTGCG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A7ZGW5

Antitoxin

• AlphaFold DB: T2HRV4