Detailed information of TA system TA080794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 94622..95147
• Replicon: plasmid unnamed1
• Accession: NZ_CP023347
• Organism: Escherichia coli strain ETEC-2265

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: T2HS38
• Locus tag: CJU64_RS27180
• Protein ID: WP_001159861.1
• Coordinates: 94622..94927 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: T2HUI2
• Locus tag: CJU64_RS27185
• Protein ID: WP_000813631.1
• Coordinates: 94929..95147 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU64_RS27150	90711..91733	+	1023	WP_001356151.1	IS21 family transposase	-
CJU64_RS27155	91730..92512	+	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
CJU64_RS27165	92734..93081	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CJU64_RS28780	93078..93458	-	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CJU64_RS27175	93815..94621	-	807	WP_000099345.1	site-specific integrase	-
CJU64_RS27180	94622..94927	-	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	Toxin
CJU64_RS27185	94929..95147	-	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CJU64_RS27195	95702..96391	+	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CJU64_RS27200	96425..97042	-	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CJU64_RS27205	97534..97764	+	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CJU64_RS27210	97761..98177	+	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	-
CJU64_RS27215	98353..99471	+	1119	Protein_123	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	csfA / cssA	1..142368	142368
	-	inside	IScluster/Tn	-	-	79439..99380	19941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11680.49 Da
• Isoelectric Point: 6.4674

>T80794 WP_001159861.1 NZ_CP023347:c94927-94622 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINMMFWGI

Nucleotide

• Length: 306 bp

>T80794 NZ_CP023347:c94927-94622 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTGCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGACTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATTGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAATGCCATTAACATGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT80794 WP_000813631.1 NZ_CP023347:c95147-94929 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMKNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT80794 NZ_CP023347:c95147-94929 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGAAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: T2HS38

Antitoxin

• AlphaFold DB: T2HUI2