Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3898228..3899028 Replicon chromosome
Accession NZ_CP023346
Organism Escherichia coli strain ETEC-2265

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag CJU64_RS20390 Protein ID WP_000342449.1
Coordinates 3898228..3898755 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag CJU64_RS20395 Protein ID WP_001277108.1
Coordinates 3898762..3899028 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU64_RS20360 3893303..3894070 - 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
CJU64_RS20365 3894067..3895344 - 1278 WP_000803798.1 branched chain amino acid ABC transporter permease LivM -
CJU64_RS20370 3895341..3896267 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CJU64_RS20375 3896315..3897424 - 1110 WP_001352761.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
CJU64_RS20385 3897848..3898231 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
CJU64_RS20390 3898228..3898755 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
CJU64_RS20395 3898762..3899028 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
CJU64_RS20400 3899178..3900281 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
CJU64_RS20410 3900553..3901407 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
CJU64_RS20415 3901652..3902710 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
CJU64_RS20420 3902703..3903371 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T80786 WP_000342449.1 NZ_CP023346:c3898755-3898228 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T80786 NZ_CP023346:c3898755-3898228 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT80786 WP_001277108.1 NZ_CP023346:c3899028-3898762 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT80786 NZ_CP023346:c3899028-3898762 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References