Detailed information of TA system TA080754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3608737..3609374
• Replicon: chromosome
• Accession: NZ_CP023341
• Organism: Bacillus velezensis strain LG37

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CMV18_RS18095
• Protein ID: WP_003156187.1
• Coordinates: 3608737..3609087 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CMV18_RS18100
• Protein ID: WP_003156188.1
• Coordinates: 3609093..3609374 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMV18_RS18055	3603777..3604379	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
CMV18_RS18060	3604379..3605167	-	789	WP_032873003.1	RNA polymerase sigma factor SigB	-
CMV18_RS18065	3605133..3605615	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
CMV18_RS18070	3605612..3605941	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CMV18_RS18075	3606005..3607012	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
CMV18_RS18080	3607024..3607425	-	402	WP_003156178.1	anti-sigma regulatory factor	-
CMV18_RS18085	3607428..3607793	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CMV18_RS18090	3607798..3608619	-	822	WP_003156182.1	STAS domain-containing protein	-
CMV18_RS18095	3608737..3609087	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CMV18_RS18100	3609093..3609374	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CMV18_RS18105	3609494..3610663	-	1170	WP_032873001.1	alanine racemase	-
CMV18_RS18110	3610780..3611787	-	1008	WP_032872999.1	outer membrane lipoprotein carrier protein LolA	-
CMV18_RS18115	3611952..3612317	-	366	WP_007609580.1	holo-ACP synthase	-
CMV18_RS18120	3612410..3613009	+	600	WP_032872997.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T80754 WP_003156187.1 NZ_CP023341:c3609087-3608737 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T80754 NZ_CP023341:c3609087-3608737 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT80754 WP_003156188.1 NZ_CP023341:c3609374-3609093 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT80754 NZ_CP023341:c3609374-3609093 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5