Detailed information of TA system TA080752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3230615..3231532
• Replicon: chromosome
• Accession: NZ_CP023320
• Organism: Bacillus velezensis strain SCGB

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CLI97_RS17090
• Protein ID: WP_057079950.1
• Coordinates: 3230786..3231532 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: CLI97_RS17085
• Protein ID: WP_003154807.1
• Coordinates: 3230615..3230785 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLI97_RS17050	3225848..3227470	+	1623	WP_094247021.1	pyocin knob domain-containing protein	-
CLI97_RS17055	3227483..3227854	+	372	WP_070081681.1	hypothetical protein	-
CLI97_RS17060	3227859..3228056	+	198	WP_071391956.1	XkdX family protein	-
CLI97_RS17065	3228113..3228874	+	762	WP_094247020.1	phage portal protein	-
CLI97_RS17070	3228926..3229189	+	264	WP_003154815.1	hemolysin XhlA family protein	-
CLI97_RS17075	3229203..3229466	+	264	WP_003154813.1	phage holin	-
CLI97_RS17080	3229480..3230358	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
CLI97_RS17085	3230615..3230785	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CLI97_RS17090	3230786..3231532	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CLI97_RS17095	3231637..3232635	-	999	WP_003154805.1	inorganic phosphate transporter	-
CLI97_RS17100	3232648..3233265	-	618	WP_003154804.1	DUF47 domain-containing protein	-
CLI97_RS17105	3233551..3234867	-	1317	WP_094247018.1	amino acid permease	-
CLI97_RS17110	3235190..3236140	+	951	WP_077722102.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T80752 WP_057079950.1 NZ_CP023320:c3231532-3230786 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T80752 NZ_CP023320:c3231532-3230786 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCAGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCAGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT80752 WP_003154807.1 NZ_CP023320:c3230785-3230615 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT80752 NZ_CP023320:c3230785-3230615 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14