Detailed information of TA system TA080751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2490021..2490658
• Replicon: chromosome
• Accession: NZ_CP023320
• Organism: Bacillus velezensis strain SCGB

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CLI97_RS13125
• Protein ID: WP_003156187.1
• Coordinates: 2490308..2490658 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CLI97_RS13120
• Protein ID: WP_003156188.1
• Coordinates: 2490021..2490302 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLI97_RS13100	2486386..2486985	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
CLI97_RS13105	2487078..2487443	+	366	WP_070081400.1	holo-ACP synthase	-
CLI97_RS13110	2487608..2488615	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
CLI97_RS13115	2488732..2489901	+	1170	WP_077721837.1	alanine racemase	-
CLI97_RS13120	2490021..2490302	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CLI97_RS13125	2490308..2490658	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CLI97_RS13130	2490776..2491597	+	822	WP_003156182.1	STAS domain-containing protein	-
CLI97_RS13135	2491602..2491967	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CLI97_RS13140	2491970..2492371	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CLI97_RS13145	2492383..2493390	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
CLI97_RS13150	2493454..2493783	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CLI97_RS13155	2493780..2494262	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CLI97_RS13160	2494228..2495016	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CLI97_RS13165	2495016..2495618	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T80751 WP_003156187.1 NZ_CP023320:2490308-2490658 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T80751 NZ_CP023320:2490308-2490658 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT80751 WP_003156188.1 NZ_CP023320:2490021-2490302 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT80751 NZ_CP023320:2490021-2490302 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5