Detailed information of TA system TA080750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 189888..190524
• Replicon: chromosome
• Accession: NZ_CP023317
• Organism: Priestia megaterium strain A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: CEQ83_RS01045
• Protein ID: WP_013055004.1
• Coordinates: 190174..190524 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: CEQ83_RS01040
• Protein ID: WP_013055003.1
• Coordinates: 189888..190169 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ83_RS01015	185254..186225	+	972	WP_033580801.1	UV DNA damage repair endonuclease UvsE	-
CEQ83_RS01020	186234..186836	-	603	WP_028411882.1	rhomboid family intramembrane serine protease	-
CEQ83_RS01025	186901..187266	+	366	WP_028411881.1	holo-ACP synthase	-
CEQ83_RS01030	187325..188383	+	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
CEQ83_RS01035	188497..189687	+	1191	WP_034327253.1	alanine racemase	-
CEQ83_RS01040	189888..190169	+	282	WP_013055003.1	hypothetical protein	Antitoxin
CEQ83_RS01045	190174..190524	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CEQ83_RS01050	190682..191518	+	837	WP_013055005.1	STAS domain-containing protein	-
CEQ83_RS01055	191521..191877	+	357	WP_013055006.1	STAS domain-containing protein	-
CEQ83_RS01060	191881..192282	+	402	WP_013055007.1	anti-sigma regulatory factor	-
CEQ83_RS01065	192296..193306	+	1011	WP_155016969.1	PP2C family protein-serine/threonine phosphatase	-
CEQ83_RS01070	193366..193698	+	333	WP_013055009.1	anti-sigma factor antagonist	-
CEQ83_RS01075	193695..194180	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
CEQ83_RS01080	194146..194940	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T80750 WP_013055004.1 NZ_CP023317:190174-190524 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T80750 NZ_CP023317:190174-190524 [Priestia megaterium]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT80750 WP_013055003.1 NZ_CP023317:189888-190169 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT80750 NZ_CP023317:189888-190169 [Priestia megaterium]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9