Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Xre(antitoxin)
Location 4842357..4843615 Replicon chromosome
Accession NZ_CP023316
Organism Pseudomonas aeruginosa strain PPF-1

Toxin (Protein)


Gene name - Uniprot ID A0A509JCW5
Locus tag B8B76_RS23015 Protein ID WP_021264413.1
Coordinates 4842357..4843112 (-) Length 252 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID V6AJU7
Locus tag B8B76_RS23020 Protein ID WP_003086329.1
Coordinates 4843247..4843615 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8B76_RS23005 4837528..4840071 + 2544 WP_023101446.1 acyl-homoserine-lactone acylase QuiP -
B8B76_RS23010 4840226..4841587 - 1362 WP_073647918.1 DNA recombination protein RmuC -
B8B76_RS23015 4842357..4843112 - 756 WP_021264413.1 RES family NAD+ phosphorylase Toxin
B8B76_RS23020 4843247..4843615 - 369 WP_003086329.1 DUF2384 domain-containing protein Antitoxin
B8B76_RS23025 4843688..4844974 - 1287 WP_003086327.1 FAD-binding oxidoreductase -
B8B76_RS23030 4845030..4846619 - 1590 WP_003123191.1 aldehyde dehydrogenase family protein -
B8B76_RS23035 4846698..4847174 + 477 WP_019486191.1 elongation factor GreAB -
B8B76_RS33320 4847177..4847452 - 276 WP_023088486.1 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(63-207)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-47)


Sequences

Toxin        


Download         Length: 252 a.a.        Molecular weight: 28217.33 Da        Isoelectric Point: 8.0554

>T80747 WP_021264413.1 NZ_CP023316:c4843112-4842357 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLQTAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAIRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Download         Length: 756 bp

>T80747 NZ_CP023316:c4843112-4842357 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGCAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATACGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13367.63 Da        Isoelectric Point: 9.9874

>AT80747 WP_003086329.1 NZ_CP023316:c4843615-4843247 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Download         Length: 369 bp

>AT80747 NZ_CP023316:c4843615-4843247 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A509JCW5

Antitoxin

Source ID Structure
AlphaFold DB V6AJU7

References