Detailed information of TA system TA080745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2932136..2933178
• Replicon: chromosome
• Accession: NZ_CP023316
• Organism: Pseudomonas aeruginosa strain PPF-1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: B8B76_RS14195
• Protein ID: WP_003153636.1
• Coordinates: 2932603..2933178 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: B8B76_RS14190
• Protein ID: WP_003050245.1
• Coordinates: 2932136..2932606 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8B76_RS14155	2927528..2928946	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
B8B76_RS14160	2928936..2929847	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
B8B76_RS14165	2929844..2930536	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
B8B76_RS14170	2930533..2930931	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
B8B76_RS14175	2930943..2931302	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
B8B76_RS14180	2931319..2931552	-	234	WP_023101201.1	TIGR03758 family integrating conjugative element protein	-
B8B76_RS14185	2931549..2931932	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
B8B76_RS14190	2932136..2932606	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
B8B76_RS14195	2932603..2933178	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
B8B76_RS14200	2933196..2934110	+	915	WP_016852809.1	AAA family ATPase	-
B8B76_RS14205	2934107..2934577	+	471	WP_003090160.1	hypothetical protein	-
B8B76_RS14210	2934574..2935074	+	501	WP_003090159.1	hypothetical protein	-
B8B76_RS14215	2935074..2935976	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
B8B76_RS14220	2936015..2936740	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2857570..2985232	127662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T80745 WP_003153636.1 NZ_CP023316:2932603-2933178 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T80745 NZ_CP023316:2932603-2933178 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT80745 WP_003050245.1 NZ_CP023316:2932136-2932606 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT80745 NZ_CP023316:2932136-2932606 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68