Detailed information of TA system TA080744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 2664664..2665217
• Replicon: chromosome
• Accession: NZ_CP023316
• Organism: Pseudomonas aeruginosa strain PPF-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q88JZ3
• Locus tag: B8B76_RS12955
• Protein ID: WP_010953434.1
• Coordinates: 2664924..2665217 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B8B76_RS12950
• Protein ID: WP_058011945.1
• Coordinates: 2664664..2664936 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8B76_RS12915	2659884..2660093	-	210	WP_058011949.1	hypothetical protein	-
B8B76_RS12920	2660107..2660604	-	498	WP_004420426.1	DNA repair protein RadC	-
B8B76_RS12925	2660576..2661526	-	951	WP_058011948.1	hypothetical protein	-
B8B76_RS12930	2661618..2662622	-	1005	WP_058011947.1	YqaJ viral recombinase family protein	-
B8B76_RS12935	2662694..2663662	-	969	WP_013970872.1	DUF932 domain-containing protein	-
B8B76_RS12940	2663760..2664089	-	330	WP_058011946.1	hypothetical protein	-
B8B76_RS12945	2664294..2664539	+	246	WP_010953432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
B8B76_RS12950	2664664..2664936	+	273	WP_058011945.1	CopG family ribbon-helix-helix protein	Antitoxin
B8B76_RS12955	2664924..2665217	+	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B8B76_RS12960	2665211..2666620	-	1410	WP_010953435.1	site-specific integrase	-
B8B76_RS12965	2666927..2668125	-	1199	Protein_2500	tyrosine-type recombinase/integrase	-
B8B76_RS33145	2668161..2668403	+	243	WP_153575148.1	hypothetical protein	-
B8B76_RS12975	2668979..2669848	+	870	Protein_2502	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2649121..2668125	19004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11155.61 Da
• Isoelectric Point: 5.9081

>T80744 WP_010953434.1 NZ_CP023316:2664924-2665217 [Pseudomonas aeruginosa]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEESPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T80744 NZ_CP023316:2664924-2665217 [Pseudomonas aeruginosa]
GTGCCACGACTGATCGTCACTGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCACGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCTTTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGTTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10359.51 Da
• Isoelectric Point: 5.5744

>AT80744 WP_058011945.1 NZ_CP023316:2664664-2664936 [Pseudomonas aeruginosa]
MATSIKIDDDLKGRIQHLACLRQRSSHWIMREAITQYVEREEARESFKQEALASWGAYQETGQHLTGQEARTWLGSWGTD
AEAELPKCHD

Nucleotide

• Length: 273 bp

>AT80744 NZ_CP023316:2664664-2664936 [Pseudomonas aeruginosa]
ATGGCCACTTCCATCAAAATTGACGACGATCTGAAAGGTCGCATTCAGCATTTGGCTTGTTTGCGCCAGCGCTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGGTGCATACCAGGAAACCGGCCAGCACTTGACCGGCCAGGAGGCTCGCACCTGGCTTGGTAGTTGGGGTACAGAT
GCTGAGGCAGAGTTACCCAAGTGCCACGACTGA

Structures

Toxin

• AlphaFold DB: A0A2W5CNE8