Detailed information of TA system TA080742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 151828..152333
• Replicon: chromosome
• Accession: NZ_CP023316
• Organism: Pseudomonas aeruginosa strain PPF-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: B8B76_RS00710
• Protein ID: WP_003083773.1
• Coordinates: 151828..152109 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A0H2ZJU8
• Locus tag: B8B76_RS00715
• Protein ID: WP_003137009.1
• Coordinates: 152106..152333 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B8B76_RS00685	147079..148428	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
B8B76_RS00690	148477..149163	+	687	WP_003083762.1	FadR family transcriptional regulator	-
B8B76_RS00695	149264..149998	+	735	WP_023107524.1	GntR family transcriptional regulator	-
B8B76_RS00700	150202..150588	+	387	WP_003083767.1	aegerolysin family protein	-
B8B76_RS00705	150620..151528	-	909	WP_031693494.1	LysR family transcriptional regulator	-
B8B76_RS00710	151828..152109	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
B8B76_RS00715	152106..152333	-	228	WP_003137009.1	CopG family ribbon-helix-helix protein	Antitoxin
B8B76_RS00720	152509..153129	-	621	WP_031693495.1	hypothetical protein	-
B8B76_RS00725	153230..153730	+	501	WP_003101228.1	LEA type 2 family protein	-
B8B76_RS00730	153803..154144	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
B8B76_RS00735	154226..155653	-	1428	WP_023092260.1	GABA permease	-
B8B76_RS00740	155822..157315	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T80742 WP_003083773.1 NZ_CP023316:c152109-151828 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T80742 NZ_CP023316:c152109-151828 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTGGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGTGCCCCGTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8526.56 Da
• Isoelectric Point: 5.5740

>AT80742 WP_003137009.1 NZ_CP023316:c152333-152106 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHAEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT80742 NZ_CP023316:c152333-152106 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAGCACGCCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A0H2ZJU8