Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 741180..741739 Replicon chromosome
Accession NZ_CP023311
Organism Vibrio anguillarum strain VIB12 isolate Sea bass

Toxin (Protein)


Gene name relE Uniprot ID F7YRQ7
Locus tag CMV05_RS19465 Protein ID WP_010320663.1
Coordinates 741461..741739 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A233HRF0
Locus tag CMV05_RS19460 Protein ID WP_017040620.1
Coordinates 741180..741464 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CMV05_RS19425 736329..736649 + 321 WP_019283194.1 IS66 family insertion sequence hypothetical protein -
CMV05_RS19430 736646..736999 + 354 WP_019283195.1 IS66 family insertion sequence element accessory protein TnpB -
CMV05_RS19435 737059..738591 + 1533 WP_096036126.1 IS66-like element ISVa11 family transposase -
CMV05_RS19440 738627..738878 + 252 Protein_723 IS30 family transposase -
CMV05_RS19445 739010..739246 + 237 WP_013867767.1 hypothetical protein -
CMV05_RS19450 739921..740454 - 534 WP_064626947.1 cysteine hydrolase -
CMV05_RS19460 741180..741464 + 285 WP_017040620.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CMV05_RS19465 741461..741739 + 279 WP_010320663.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
CMV05_RS19490 743155..744323 - 1169 Protein_728 ISL3-like element ISVa10 family transposase -
CMV05_RS19495 744485..744841 - 357 Protein_729 transporter substrate-binding domain-containing protein -
CMV05_RS19500 745072..745497 - 426 Protein_730 efflux RND transporter permease subunit -
CMV05_RS19505 745504..746535 - 1032 WP_026027704.1 IS630-like element ISVa15 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 682266..761421 79155
- inside Integron - - 739010..742871 3861


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-44)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10878.58 Da        Isoelectric Point: 4.3430

>T80715 WP_010320663.1 NZ_CP023311:741461-741739 [Vibrio anguillarum]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDDIIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVEGSIIRIM
RVLHQKQKFPTD

Download         Length: 279 bp

>T80715 NZ_CP023311:741461-741739 [Vibrio anguillarum]
ATGATTTTATGGGAAGAGGAATCGCTAAATGATCGTGAGAAGATCTTTGAGTTTCTTTATGACTTTAACCCTGATGCGGC
TGAAAAAACTGATGACATTATTGAGGCTAAAGTAGAAAACTTACTCGAACAACCATTGATGGGTGTTCAACGGGATGGAA
TTCGAGGACGATTACTCATTATTCCCGAAATCTCAATGATCGTTTCGTACTGGGTAGAAGGTTCAATTATTCGCATAATG
CGAGTTCTACACCAGAAGCAGAAATTCCCTACCGATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10957.29 Da        Isoelectric Point: 9.2173

>AT80715 WP_017040620.1 NZ_CP023311:741180-741464 [Vibrio anguillarum]
MDTRIQFRVDDEIKRLAQQMAESQGRTLSDACRELTEQMAEQQRKTLSHDAWLTEQINLAFEKFDSGKSSFVEHNSAKAR
MAERKAKIRNRGQQ

Download         Length: 285 bp

>AT80715 NZ_CP023311:741180-741464 [Vibrio anguillarum]
ATGGACACTAGAATTCAATTTCGTGTTGATGACGAAATTAAACGTTTAGCCCAACAAATGGCTGAGAGCCAAGGTCGCAC
ACTCAGCGATGCTTGCCGTGAACTTACTGAACAAATGGCTGAGCAACAAAGAAAAACATTATCTCATGACGCTTGGTTAA
CTGAACAAATAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTTCATTTGTTGAACATAATTCAGCTAAAGCACGA
ATGGCTGAACGAAAGGCCAAAATTCGTAATCGAGGCCAGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2IQF3

Antitoxin

Source ID Structure
AlphaFold DB A0A233HRF0

References