Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 700239..701005 Replicon chromosome
Accession NZ_CP023311
Organism Vibrio anguillarum strain VIB12 isolate Sea bass

Toxin (Protein)


Gene name - Uniprot ID A0A290PTK1
Locus tag CMV05_RS19185 Protein ID WP_017045788.1
Coordinates 700508..701005 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A290Q6J3
Locus tag CMV05_RS19180 Protein ID WP_019283269.1
Coordinates 700239..700511 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CMV05_RS19140 696101..696454 - 354 WP_019283195.1 IS66 family insertion sequence element accessory protein TnpB -
CMV05_RS19145 696451..696771 - 321 WP_096036125.1 IS66 family insertion sequence hypothetical protein -
CMV05_RS23720 696851..697012 + 162 Protein_668 IS30 family transposase -
CMV05_RS19150 697362..697763 - 402 Protein_669 hypothetical protein -
CMV05_RS19155 697934..698338 - 405 WP_017039704.1 DUF1801 domain-containing protein -
CMV05_RS19160 698476..699012 - 537 WP_095470047.1 nucleotidyltransferase family protein -
CMV05_RS19175 700038..700199 - 162 WP_096037229.1 DUF3265 domain-containing protein -
CMV05_RS19180 700239..700511 + 273 WP_019283269.1 DUF1778 domain-containing protein Antitoxin
CMV05_RS19185 700508..701005 + 498 WP_017045788.1 GNAT family N-acetyltransferase Toxin
CMV05_RS19190 701246..702778 - 1533 WP_096036126.1 IS66-like element ISVa11 family transposase -
CMV05_RS19195 702838..703191 - 354 WP_019283195.1 IS66 family insertion sequence element accessory protein TnpB -
CMV05_RS19200 703188..703283 - 96 Protein_677 IS66 family insertion sequence element accessory protein TnpB -
CMV05_RS19210 704828..705079 - 252 WP_096037036.1 IS66 family insertion sequence hypothetical protein -
CMV05_RS19215 705351..705464 + 114 WP_096037037.1 DUF3265 domain-containing protein -
CMV05_RS19220 705490..705915 + 426 WP_045571622.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 682266..761421 79155
- inside Integron - - 697038..702778 5740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18524.30 Da        Isoelectric Point: 9.2813

>T80714 WP_017045788.1 NZ_CP023311:700508-701005 [Vibrio anguillarum]
MMKTVLLDKDKHDRNRFNCGIDALNNYLKVMASQQAKKDNTRTFVLEDENNTSLIIGFYTLTMTPIDLKTLPDKLQKKHQ
SSTSGGLIARLAIDDSYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFKAFQDAENKLFITISDI
RASLG

Download         Length: 498 bp

>T80714 NZ_CP023311:700508-701005 [Vibrio anguillarum]
ATGATGAAAACGGTATTACTCGATAAAGATAAACACGACAGAAACCGATTCAACTGCGGAATTGATGCGCTTAATAACTA
TTTGAAAGTCATGGCAAGTCAGCAAGCTAAAAAAGATAATACCCGAACATTTGTTTTAGAGGATGAAAATAACACCTCAC
TTATCATCGGTTTTTACACTTTAACAATGACACCCATTGACCTAAAAACATTGCCTGATAAGTTACAGAAAAAGCATCAG
TCATCAACTTCTGGCGGACTCATTGCTCGCTTAGCTATCGATGATAGCTATAAAGGTAAAGGATTTGGCGAGTGGTTACT
TATCGATGCTCTACGAAAACTACTAGCAGCTAGTGATAGCGTGGCATTTCCCGTCGTTATTGTTGACGCAAAAGATGGCG
CCAAACATTTCTATGAGCGCTATGGTTTTAAAGCGTTTCAAGATGCAGAAAATAAGCTATTTATCACCATTTCAGATATT
AGGGCAAGTTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9917.47 Da        Isoelectric Point: 9.8130

>AT80714 WP_019283269.1 NZ_CP023311:700239-700511 [Vibrio anguillarum]
MATTLPRITARVDVDTQNLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQTLKLSQTDAILLMEALDKPTTLNQKLKT
AAERYETRTQ

Download         Length: 273 bp

>AT80714 NZ_CP023311:700239-700511 [Vibrio anguillarum]
ATGGCTACCACTTTACCTCGTATCACTGCGAGAGTTGATGTCGATACACAAAACTTATTAACCAAAGCGGCTGCTCTCGC
TGGCATGTCTAGTATTAACTCATTTGTACTCAATGCTGCAATCGAAAAAGCAAAGCAAGTGATTGAACGCGAACAAACAC
TAAAATTAAGCCAAACAGACGCCATTTTACTTATGGAAGCTCTCGATAAACCAACAACATTAAATCAAAAGCTTAAAACT
GCCGCCGAACGCTATGAGACCAGAACCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A290PTK1

Antitoxin

Source ID Structure
AlphaFold DB A0A290Q6J3

References