Detailed information of TA system TA080713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 679355..679883
• Replicon: chromosome
• Accession: NZ_CP023311
• Organism: Vibrio anguillarum strain VIB12 isolate Sea bass

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CMV05_RS19025
• Protein ID: WP_096037028.1
• Coordinates: 679355..679642 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F7YRA5
• Locus tag: CMV05_RS19030
• Protein ID: WP_013868555.1
• Coordinates: 679632..679883 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMV05_RS18985	674507..675427	+	921	WP_096037026.1	LysR family transcriptional regulator	-
CMV05_RS18995	676087..676527	-	441	WP_088733311.1	hypothetical protein	-
CMV05_RS19010	677287..677619	+	333	WP_069573255.1	SEC-C domain-containing protein	-
CMV05_RS23705	678182..678304	-	123	Protein_642	DUF3265 domain-containing protein	-
CMV05_RS19015	678327..678617	-	291	WP_026028227.1	YkgJ family cysteine cluster protein	-
CMV05_RS19025	679355..679642	-	288	WP_096037028.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CMV05_RS19030	679632..679883	-	252	WP_013868555.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CMV05_RS19035	680180..680428	-	249	WP_026028286.1	DUF3297 family protein	-
CMV05_RS19045	680747..680977	+	231	WP_013868597.1	PAS factor family protein	-
CMV05_RS19050	681097..681303	-	207	WP_017048743.1	DUF1289 domain-containing protein	-
CMV05_RS19055	682266..682586	+	321	WP_019283194.1	IS66 family insertion sequence hypothetical protein	-
CMV05_RS19060	682583..682936	+	354	WP_019283195.1	IS66 family insertion sequence element accessory protein TnpB	-
CMV05_RS19065	682996..684528	+	1533	WP_096036126.1	IS66-like element ISVa11 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	682266..761421	79155
	-	inside	Integron	-	-	674507..694490	19983

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (11-70)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11078.09 Da
• Isoelectric Point: 10.5506

>T80713 WP_096037028.1 NZ_CP023311:c679642-679355 [Vibrio anguillarum]
MTYKLKFLPAAKKEWSKLAPPIQSQFKKKLKERLENPHLPSSKLRGYDSVYKIKLRTAGYRLAYEVIDDEIVVYVLAIGK
RDKDAVYKKLASRFS

Nucleotide

• Length: 288 bp

>T80713 NZ_CP023311:c679642-679355 [Vibrio anguillarum]
ATGACTTATAAGCTAAAATTTCTACCGGCTGCCAAGAAAGAGTGGAGTAAGTTAGCTCCACCAATTCAAAGTCAGTTCAA
AAAGAAGCTTAAAGAGCGTCTGGAAAACCCGCATCTTCCTTCTTCTAAACTGCGTGGCTATGACTCAGTTTATAAGATCA
AACTACGTACAGCAGGCTATCGCTTAGCCTATGAAGTCATTGATGATGAAATCGTGGTGTACGTGTTAGCTATTGGAAAA
CGAGATAAAGATGCAGTGTACAAAAAGTTAGCATCAAGGTTCTCGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9154.36 Da
• Isoelectric Point: 4.0414

>AT80713 WP_013868555.1 NZ_CP023311:c679883-679632 [Vibrio anguillarum]
MRQVLADCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEFLMDMLDDYELAKLVESRRGELSEAVEVNI
DDL

Nucleotide

• Length: 252 bp

>AT80713 NZ_CP023311:c679883-679632 [Vibrio anguillarum]
ATGAGACAGGTTTTAGCTGATTGTTCTGCAAGTATTTCTGAGTTAAAGAAAAATCCAACCGCTTTACTAAACGAAGCTGA
TGGTTCTGCCATTGCTATTCTAAACCACAATAAGCCAGCAGCTTATCTTGTCCCAGCAGAAACTTATGAGTTTCTAATGG
ATATGCTCGATGACTATGAGCTTGCTAAACTAGTTGAAAGTCGCCGTGGTGAATTGTCTGAAGCGGTGGAAGTAAATATC
GATGACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7X8TZB8