Detailed information of TA system TA080712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 558423..558988
• Replicon: chromosome
• Accession: NZ_CP023311
• Organism: Vibrio anguillarum strain VIB12 isolate Sea bass

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F2IXR1
• Locus tag: CMV05_RS18215
• Protein ID: WP_017049660.1
• Coordinates: 558701..558988 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CMV05_RS18210
• Protein ID: WP_164996843.1
• Coordinates: 558423..558704 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CMV05_RS18185	553793..553942	+	150	WP_096037221.1	DUF3265 domain-containing protein	-
CMV05_RS18190	553981..554271	+	291	WP_064626913.1	hypothetical protein	-
CMV05_RS23635	554327..554437	+	111	WP_107489896.1	DUF3265 domain-containing protein	-
CMV05_RS18200	555224..556462	+	1239	WP_086558351.1	IS256-like element ISVa19 family transposase	-
CMV05_RS18210	558423..558704	+	282	WP_164996843.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CMV05_RS18215	558701..558988	+	288	WP_017049660.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CMV05_RS18220	558998..559108	+	111	WP_088129430.1	DUF3265 domain-containing protein	-
CMV05_RS18225	559169..559534	+	366	WP_096036984.1	hypothetical protein	-
CMV05_RS18230	559567..560598	+	1032	WP_026027704.1	IS630-like element ISVa15 family transposase	-
CMV05_RS18235	560836..561300	-	465	WP_096036985.1	GNAT family N-acetyltransferase	-
CMV05_RS18240	561789..561881	-	93	WP_096037222.1	DUF3265 domain-containing protein	-
CMV05_RS18245	561910..563529	-	1620	WP_086558331.1	IS1634-like element ISVa17 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	536908..563529	26621
	-	inside	Integron	-	-	529673..559534	29861

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10929.71 Da
• Isoelectric Point: 5.6023

>T80712 WP_017049660.1 NZ_CP023311:558701-558988 [Vibrio anguillarum]
MKVVWSPLALQKLGDAAEFIALDNPSAAEKWVNDVFDKTELLGSMPEMGRMVPEMPHTNYREIIFGHYRIIYSLGLEIRV
LTVRNCRQLLTEHDV

Nucleotide

• Length: 288 bp

>T80712 NZ_CP023311:558701-558988 [Vibrio anguillarum]
ATGAAAGTAGTTTGGTCACCTCTAGCGTTACAAAAACTTGGTGATGCCGCAGAGTTTATTGCTTTGGATAACCCCTCAGC
TGCAGAAAAGTGGGTGAATGATGTGTTCGACAAAACGGAACTGCTCGGCTCAATGCCAGAAATGGGCCGCATGGTTCCTG
AAATGCCTCATACGAACTACCGTGAAATAATTTTTGGCCATTACCGCATTATTTATAGTTTGGGCCTCGAAATACGTGTT
CTAACAGTCCGTAACTGTCGTCAATTGCTCACGGAACATGATGTGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10361.93 Da
• Isoelectric Point: 5.1548

>AT80712 WP_164996843.1 NZ_CP023311:558423-558704 [Vibrio anguillarum]
MSRIHLDQDIQPLSEFRAGVASFIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLAAGLGVSN
EDARSQILGRIIK

Nucleotide

• Length: 282 bp

>AT80712 NZ_CP023311:558423-558704 [Vibrio anguillarum]
ATGAGCCGTATTCACCTTGATCAAGATATTCAGCCTTTGTCTGAGTTCCGTGCTGGCGTTGCATCATTTATCAAACAGAT
CAATGAAACTCGTCGACCATTAGTTATTACACAACGAGGTAAAGGTGTAGCCGTTGTTCTTGACGTTGCGGAGTATGAAG
CAATGCAAGAGAAAATCGAATTACTTGAAGAAATGCGTACTGCAGAAGCTCAATTGGCTGCAGGTTTGGGTGTATCAAAC
GAAGATGCTCGTTCACAAATTCTGGGACGCATCATCAAATGA

Structures

Toxin

• AlphaFold DB: A0A0F2IXR1