Detailed information of TA system TA080711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 1528798..1529312
• Replicon: chromosome
• Accession: NZ_CP023298
• Organism: Actinoplanes sp. SE50

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ACWT_RS06940
• Protein ID: WP_014688436.1
• Coordinates: 1528798..1529058 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: G8S4Y5
• Locus tag: ACWT_RS06945
• Protein ID: WP_014688437.1
• Coordinates: 1529055..1529312 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACWT_RS06925	1524325..1526415	+	2091	WP_080127737.1	UvrD-helicase domain-containing protein	-
ACWT_RS06930	1526853..1527980	+	1128	WP_197688122.1	hypothetical protein	-
ACWT_RS06935	1528038..1528775	+	738	WP_014688435.1	hypothetical protein	-
ACWT_RS06940	1528798..1529058	-	261	WP_014688436.1	Txe/YoeB family addiction module toxin	Toxin
ACWT_RS06945	1529055..1529312	-	258	WP_014688437.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
ACWT_RS06955	1530771..1531172	-	402	WP_043510958.1	hypothetical protein	-
ACWT_RS41970	1531282..1531503	-	222	WP_155123447.1	hypothetical protein	-
ACWT_RS06960	1531692..1531943	+	252	WP_155123448.1	hypothetical protein	-
ACWT_RS06965	1532043..1532753	-	711	WP_014688442.1	SDR family oxidoreductase	-
ACWT_RS06970	1532812..1533174	+	363	WP_014688443.1	MerR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-70)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10231.53 Da
• Isoelectric Point: 8.0720

>T80711 WP_014688436.1 NZ_CP023298:c1529058-1528798 [Actinoplanes sp. SE50]
VKLSWTDHAWDDYLYRQTQDRRTLKRINALIADIKRDPDGQGIGKPEILRNNLAGLRSRRIDDEHRMVYAVETNEITIIS
CRYHHE

Nucleotide

• Length: 261 bp

>T80711 NZ_CP023298:c1529058-1528798 [Actinoplanes sp. SE50]
GTGAAACTCAGCTGGACCGACCACGCCTGGGACGACTACCTGTACCGGCAGACTCAGGACCGCAGAACCCTCAAACGCAT
CAACGCCCTGATCGCCGACATCAAACGTGATCCCGACGGTCAGGGCATCGGCAAACCGGAGATCCTCCGAAACAACCTCG
CGGGACTCCGGTCCCGGCGCATCGACGACGAACACCGCATGGTGTACGCCGTCGAGACCAACGAGATCACCATCATCTCC
TGCCGATACCATCACGAATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9598.77 Da
• Isoelectric Point: 5.1667

>AT80711 WP_014688437.1 NZ_CP023298:c1529312-1529055 [Actinoplanes sp. SE50]
VTSAISASEARKSLFPLIERVNLDHTPIEIVSKRGNAVLVSKEDWDSIMETNYLLRSPANAKRLTESVEQWRAGRATERE
FGPEE

Nucleotide

• Length: 258 bp

>AT80711 NZ_CP023298:c1529312-1529055 [Actinoplanes sp. SE50]
GTGACTTCAGCGATCAGTGCCAGCGAGGCTCGCAAGTCCCTCTTTCCGCTCATCGAACGAGTCAACCTGGACCACACTCC
GATCGAAATCGTCTCCAAGCGCGGCAACGCCGTCCTGGTGAGCAAGGAGGACTGGGACTCGATCATGGAAACCAACTACC
TGCTTCGATCCCCCGCCAACGCCAAGCGCCTCACTGAGAGCGTCGAGCAATGGCGAGCGGGACGAGCCACGGAGCGCGAG
TTCGGCCCCGAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: G8S4Y5