Detailed information of TA system TA080710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 37670..38259
• Replicon: plasmid pP2
• Accession: NZ_CP023287
• Organism: Xanthomonas citri pv. citri strain 03-1638-1-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: CLM98_RS24725
• Protein ID: WP_015463824.1
• Coordinates: 37670..38002 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: CLM98_RS24730
• Protein ID: WP_011052949.1
• Coordinates: 38002..38259 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLM98_RS25535	36150..36371	-	222	WP_011052944.1	hypothetical protein	-
CLM98_RS24715	36661..37197	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
CLM98_RS24720	37187..37399	-	213	WP_011052947.1	hypothetical protein	-
CLM98_RS25380	37396..37647	-	252	WP_125459571.1	hypothetical protein	-
CLM98_RS24725	37670..38002	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CLM98_RS24730	38002..38259	-	258	WP_011052949.1	antitoxin	Antitoxin
CLM98_RS24735	38465..39067	+	603	WP_011052950.1	recombinase family protein	-
CLM98_RS24740	39051..39355	+	305	Protein_48	Tn3 family transposase	-
CLM98_RS24745	39522..39728	-	207	Protein_49	Tn3 family transposase	-
CLM98_RS24750	39773..40342	-	570	Protein_50	UPF0149 family protein	-
CLM98_RS24755	40361..41575	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
CLM98_RS24760	41649..41831	-	183	Protein_52	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99153	99153

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T80710 WP_015463824.1 NZ_CP023287:c38002-37670 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T80710 NZ_CP023287:c38002-37670 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT80710 WP_011052949.1 NZ_CP023287:c38259-38002 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT80710 NZ_CP023287:c38259-38002 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6