Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 3219356..3221095 Replicon chromosome
Accession NZ_CP023284
Organism Variovorax boronicumulans strain J1

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag CKY39_RS14750 Protein ID WP_095744976.1
Coordinates 3219356..3220225 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A554S243
Locus tag CKY39_RS14755 Protein ID WP_070060330.1
Coordinates 3220262..3221095 (+) Length 278 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKY39_RS14725 3214455..3215423 + 969 WP_157755232.1 2-dehydropantoate 2-reductase -
CKY39_RS14730 3215478..3216737 + 1260 WP_095744974.1 D-amino acid dehydrogenase -
CKY39_RS14735 3216929..3217912 + 984 WP_062474573.1 tripartite tricarboxylate transporter substrate binding protein -
CKY39_RS14740 3217990..3218748 + 759 WP_185993640.1 SDR family oxidoreductase -
CKY39_RS14745 3218792..3219328 + 537 WP_062474577.1 cupin domain-containing protein -
CKY39_RS14750 3219356..3220225 + 870 WP_095744976.1 alpha/beta hydrolase Toxin
CKY39_RS14755 3220262..3221095 + 834 WP_070060330.1 IclR family transcriptional regulator Antitoxin
CKY39_RS14760 3221116..3221955 + 840 WP_095744977.1 SDR family oxidoreductase -
CKY39_RS14765 3221948..3223645 + 1698 WP_062474586.1 thiamine pyrophosphate-binding protein -
CKY39_RS14770 3223666..3224466 + 801 WP_062474588.1 aspartate dehydrogenase -
CKY39_RS14775 3224475..3225950 + 1476 WP_095744978.1 aldehyde dehydrogenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(47-281)

Antitoxin

(139-258)


Sequences


Toxin        


Download         Length: 290 a.a.        Molecular weight: 29898.21 Da        Isoelectric Point: 6.0184

>T80703 WP_095744976.1 NZ_CP023284:3219356-3220225 [Variovorax boronicumulans]
MSTVDISAQTLPASELARLDARFPARDVAVGGGAVVSVRECGQGPVIVCLHGIGSGAASWLDTAALLAPQACLIAWDAPG
YGRSTPLASAAPTAADYAARLEEMLDALSIRSCVLVGHSLGAIVAASAARSESSASERIRRLVLVSPAIGYGAAGRIDAR
AKVRAERLATLDDLGIAGMAAKRAGRLVSDGASEHARQWVRWNMARLNDHGYRQAVELLCNADLLADLPPPMPVRVACGA
LDVVTPPTACEEVARRCGVPLELVDDAGHAGYVEQPQAVAALLRESLAG

Download         Length: 870 bp

>T80703 NZ_CP023284:3219356-3220225 [Variovorax boronicumulans]
ATGAGCACCGTCGACATCTCCGCGCAGACCCTGCCGGCTTCCGAGCTGGCGCGACTCGACGCGCGCTTTCCGGCCCGCGA
CGTCGCGGTGGGCGGCGGCGCGGTGGTGTCGGTGCGCGAATGCGGGCAGGGCCCGGTCATCGTGTGCCTGCACGGCATCG
GCTCGGGCGCGGCCTCGTGGCTCGACACCGCGGCGCTGCTGGCGCCGCAGGCCTGCCTCATCGCCTGGGATGCACCGGGC
TACGGCCGCTCGACGCCGCTGGCCTCGGCCGCGCCGACCGCCGCGGACTACGCCGCACGCCTTGAAGAAATGCTCGACGC
GCTGTCGATCCGCTCCTGCGTGCTCGTCGGCCATTCGCTGGGCGCCATCGTGGCCGCGTCGGCTGCGCGCAGCGAGTCGA
GCGCCAGTGAGCGCATCCGCCGCCTCGTGCTCGTGAGCCCCGCCATCGGCTACGGCGCCGCCGGGCGCATCGACGCCCGC
GCCAAGGTGCGCGCCGAACGCCTGGCCACGCTCGACGACCTCGGCATTGCCGGCATGGCCGCCAAGCGCGCGGGCCGGCT
CGTGTCCGACGGTGCCAGCGAACACGCCCGCCAGTGGGTGCGTTGGAACATGGCGCGTCTGAACGACCACGGCTACCGCC
AGGCCGTCGAGCTGCTGTGCAACGCCGACCTGCTGGCCGACCTGCCGCCGCCGATGCCCGTGCGCGTGGCCTGCGGCGCG
CTCGACGTCGTCACGCCGCCCACCGCCTGCGAAGAAGTCGCGCGCCGCTGCGGCGTGCCCCTCGAATTGGTGGACGATGC
GGGTCACGCCGGCTATGTGGAACAACCGCAGGCCGTCGCCGCCTTGCTGCGCGAATCGCTCGCCGGCTGA

Antitoxin


Download         Length: 278 a.a.        Molecular weight: 30605.96 Da        Isoelectric Point: 5.7705

>AT80703 WP_070060330.1 NZ_CP023284:3220262-3221095 [Variovorax boronicumulans]
MATDNEVMEEGADRYNVPALERGLRVLCEFSRESRTLSAPELARRFDLPRSTVFRLLTTLENMGFLERAEGGRDYRLGLA
VLRLGFEYLASLELTQLGTPLLNRLCDELRTPCNLVVRDGRSIVYVAKVAPATPFASSVTVGTRLPAHATVLGRILLEDL
TLPQLRALYPEDKLETFSPSTPKTVNELFDMVQADRQRGYVLGEGFFESNISTIAAPVRDHSGHIVAALGATITSGHIDE
NRMDEMVQRVRGTADEISGLLNYSPARTSKVVPLRSA

Download         Length: 834 bp

>AT80703 NZ_CP023284:3220262-3221095 [Variovorax boronicumulans]
ATGGCCACCGACAACGAAGTGATGGAAGAGGGCGCGGACCGCTACAACGTGCCCGCGCTGGAGCGCGGCCTGCGCGTGCT
GTGCGAGTTCAGCCGCGAGAGCCGCACGCTGTCCGCGCCCGAGCTGGCGCGCCGCTTCGACCTGCCGCGCTCGACCGTCT
TCCGCCTGCTCACCACGCTGGAGAACATGGGTTTTCTCGAGCGCGCCGAGGGCGGACGCGACTACCGCCTGGGCCTGGCC
GTGCTGCGCCTGGGCTTCGAATACCTGGCCTCGCTGGAACTCACGCAACTGGGCACGCCGCTGCTCAACCGCCTGTGCGA
CGAGCTGCGCACGCCCTGCAACCTGGTGGTGCGCGACGGCCGCTCGATCGTCTACGTCGCCAAGGTGGCACCGGCCACGC
CCTTCGCCAGCTCGGTCACGGTGGGTACGCGCCTGCCGGCCCACGCCACGGTGCTGGGCCGCATCCTGCTGGAAGACCTC
ACGCTGCCGCAGCTGCGCGCGCTGTACCCCGAAGACAAGCTCGAGACCTTCTCGCCGAGCACGCCCAAGACCGTGAACGA
GCTCTTCGACATGGTGCAGGCCGACCGCCAGCGCGGCTATGTGCTGGGCGAGGGCTTCTTCGAATCGAACATCTCGACCA
TCGCCGCACCGGTGCGCGACCACAGCGGCCACATCGTGGCGGCACTGGGCGCCACCATCACCTCGGGCCACATCGACGAG
AACCGCATGGACGAGATGGTGCAGCGCGTGCGCGGCACCGCCGACGAGATCTCGGGCCTGCTCAATTACTCGCCCGCGCG
CACCAGCAAGGTGGTGCCGCTGCGCAGCGCCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A554S243

References