Detailed information of TA system TA080699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 83744..84339
• Replicon: chromosome
• Accession: NZ_CP023278
• Organism: Nostoc sp. CENA543

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CLI64_RS00385
• Protein ID: WP_103135385.1
• Coordinates: 83983..84339 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CLI64_RS00380
• Protein ID: WP_103135384.1
• Coordinates: 83744..83983 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLI64_RS00360	80225..80686	+	462	Protein_69	nitrogenase reductase	-
CLI64_RS00365	80826..82268	+	1443	WP_103135381.1	nitrogenase molybdenum-iron protein alpha chain	-
CLI64_RS00370	82490..83137	-	648	WP_103135382.1	EcsC family protein	-
CLI64_RS00375	83405..83665	-	261	WP_103135383.1	hypothetical protein	-
CLI64_RS00380	83744..83983	+	240	WP_103135384.1	CopG family transcriptional regulator	Antitoxin
CLI64_RS00385	83983..84339	+	357	WP_103135385.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CLI64_RS00390	84562..84750	-	189	WP_103135386.1	hypothetical protein	-
CLI64_RS00395	84878..85261	+	384	WP_103135387.1	hypothetical protein	-
CLI64_RS00400	85433..86035	-	603	WP_103135388.1	hypothetical protein	-
CLI64_RS00405	86194..88380	-	2187	WP_103135389.1	DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-114)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13379.38 Da
• Isoelectric Point: 6.9777

>T80699 WP_103135385.1 NZ_CP023278:83983-84339 [Nostoc sp. CENA543]
MPNGVLTYRRGEIRWVSLDPTVGAEVQKTRSCLIVQNDVMNQYGSLTIVMPFRPGTKKAPYVVNVKATTNNGLDQDRYID
VAQIRSVDHSRILGLIGILEDEYWEKIRAALDIVLNFE

Nucleotide

• Length: 357 bp

>T80699 NZ_CP023278:83983-84339 [Nostoc sp. CENA543]
ATGCCTAACGGTGTTTTAACTTATCGACGAGGGGAAATTCGCTGGGTATCACTTGATCCAACCGTGGGTGCAGAAGTTCA
AAAAACACGTTCTTGCCTAATAGTGCAGAATGATGTGATGAATCAATATGGTTCTTTAACTATAGTGATGCCATTTCGAC
CAGGAACTAAAAAAGCCCCTTATGTAGTGAATGTCAAAGCTACAACAAACAATGGTTTAGACCAAGACCGCTATATTGAT
GTTGCACAAATTCGTAGTGTCGATCATAGTCGAATATTAGGGTTAATAGGTATTTTGGAAGATGAATATTGGGAAAAAAT
TCGTGCTGCGCTAGATATAGTTTTGAATTTTGAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8522.55 Da
• Isoelectric Point: 4.1064

>AT80699 WP_103135384.1 NZ_CP023278:83744-83983 [Nostoc sp. CENA543]
MAKKVTITLDDEILAFIDRQAALAGDTPNRSGYVNAVLAEHRRAVLEAEIIAALKEDNENPEYQAEIAAWGAVVGDGIE

Nucleotide

• Length: 240 bp

>AT80699 NZ_CP023278:83744-83983 [Nostoc sp. CENA543]
TTGGCTAAAAAAGTTACTATTACCCTTGATGATGAGATTCTGGCTTTTATTGACCGACAGGCTGCATTAGCTGGAGATAC
TCCCAACCGGAGTGGCTATGTTAATGCTGTTTTAGCAGAACATCGTCGCGCGGTTTTAGAAGCAGAAATAATTGCAGCAC
TCAAAGAAGATAATGAAAACCCTGAATATCAGGCAGAAATAGCAGCTTGGGGTGCCGTTGTGGGAGATGGTATTGAATAA