Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 703534..704119 Replicon chromosome
Accession NZ_CP023276
Organism Polynucleobacter difficilis strain AM-8B5

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag AOC34_RS03620 Protein ID WP_108468813.1
Coordinates 703937..704119 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AOC34_RS03615 Protein ID WP_108468812.1
Coordinates 703534..703926 (-) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOC34_RS03585 698675..699616 + 942 WP_108468808.1 YicC family protein -
AOC34_RS03590 699621..700253 + 633 WP_108468809.1 guanylate kinase -
AOC34_RS03595 700299..700502 + 204 WP_108468810.1 DNA-directed RNA polymerase subunit omega -
AOC34_RS03600 700547..702907 + 2361 WP_199908341.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
AOC34_RS03605 702904..703404 - 501 WP_108468811.1 transcription elongation factor GreB -
AOC34_RS03615 703534..703926 - 393 WP_108468812.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOC34_RS03620 703937..704119 - 183 WP_108468813.1 type II toxin-antitoxin system HicA family toxin Toxin
AOC34_RS03625 704284..705438 + 1155 WP_108468814.1 ABC transporter permease -
AOC34_RS03630 705438..706193 + 756 WP_199908316.1 ATP-binding cassette domain-containing protein -
AOC34_RS03635 706190..707116 + 927 WP_108468816.1 MCE family protein -
AOC34_RS03640 707113..707796 + 684 WP_108468817.1 membrane integrity-associated transporter subunit PqiC -
AOC34_RS03645 707883..708668 + 786 WP_108468818.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6789.12 Da        Isoelectric Point: 11.1504

>T80698 WP_108468813.1 NZ_CP023276:c704119-703937 [Polynucleobacter difficilis]
MNSKELLKLLERDGWIMRNSKGSHHIFKHPVKTGHITVPHPKKDLGVGLVQKLLKQAGIK

Download         Length: 183 bp

>T80698 NZ_CP023276:c704119-703937 [Polynucleobacter difficilis]
ATGAATAGCAAAGAACTCCTCAAGTTGTTAGAGAGGGATGGTTGGATTATGCGTAACTCAAAAGGTTCCCATCATATTTT
TAAGCATCCCGTTAAAACCGGTCACATTACGGTGCCACACCCCAAAAAGGATCTCGGCGTTGGTTTAGTTCAAAAGCTAC
TAAAACAAGCAGGAATTAAATAA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 14045.03 Da        Isoelectric Point: 4.4617

>AT80698 WP_108468812.1 NZ_CP023276:c703926-703534 [Polynucleobacter difficilis]
MKYPIAIEPGNDTNAWGVIVPDLPGCFSAADSGIDEAIDNAKQAIELWIEAALDDNQDVPRPSSINALQKKKEFKGMIWA
IAEIDPALLSNEIERVNISLPKRVLARLDAKAKSAGENRSGFIAQMAISA

Download         Length: 393 bp

>AT80698 NZ_CP023276:c703926-703534 [Polynucleobacter difficilis]
ATGAAATATCCAATCGCAATAGAGCCAGGGAACGATACCAATGCGTGGGGCGTTATCGTGCCTGACTTACCAGGTTGCTT
TTCGGCTGCGGATAGCGGTATTGATGAGGCAATCGACAATGCAAAACAAGCCATTGAATTGTGGATCGAAGCTGCCTTAG
ATGACAATCAAGATGTGCCAAGACCAAGTTCGATTAATGCCCTGCAGAAGAAAAAAGAATTTAAGGGAATGATTTGGGCC
ATTGCCGAAATTGATCCTGCCCTTCTATCTAATGAAATCGAGCGTGTGAATATCTCCTTGCCAAAACGAGTGCTAGCAAG
GTTGGATGCAAAAGCAAAGTCAGCGGGAGAAAATCGCTCTGGGTTTATTGCCCAAATGGCAATCAGCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References