Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-ChpS
Location 317247..317827 Replicon chromosome
Accession NZ_CP023276
Organism Polynucleobacter difficilis strain AM-8B5

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag AOC34_RS01655 Protein ID WP_108468471.1
Coordinates 317247..317573 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag AOC34_RS01660 Protein ID WP_108468472.1
Coordinates 317573..317827 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOC34_RS01625 313543..313746 + 204 WP_159074773.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
AOC34_RS01630 313743..314390 + 648 WP_159074774.1 hypothetical protein -
AOC34_RS01635 314423..314827 + 405 WP_159074775.1 hypothetical protein -
AOC34_RS01640 314776..315396 + 621 WP_159074776.1 GspH/FimT family pseudopilin -
AOC34_RS01645 315415..316551 + 1137 WP_108468469.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
AOC34_RS01650 316620..317240 + 621 WP_108468470.1 riboflavin synthase -
AOC34_RS01655 317247..317573 - 327 WP_108468471.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AOC34_RS01660 317573..317827 - 255 WP_108468472.1 antitoxin Antitoxin
AOC34_RS01665 318005..318766 - 762 WP_108468473.1 7-cyano-7-deazaguanine synthase QueC -
AOC34_RS01670 318794..319510 - 717 WP_108468474.1 tol-pal system protein YbgF -
AOC34_RS01675 319526..320026 - 501 WP_108468475.1 peptidoglycan-associated lipoprotein Pal -
AOC34_RS01680 320121..321416 - 1296 WP_108468476.1 Tol-Pal system protein TolB -
AOC34_RS01685 321421..322275 - 855 WP_108468477.1 cell envelope integrity protein TolA -
AOC34_RS01690 322311..322739 - 429 WP_108468478.1 ExbD/TolR family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11608.65 Da        Isoelectric Point: 10.1502

>T80697 WP_108468471.1 NZ_CP023276:c317573-317247 [Polynucleobacter difficilis]
MNRGDIYLVSLDPTAGHEQKGMRPVLVVSPDAFNKLTKAPIVVPITSGGSFARLSGFTVDLIGSKTMGVVRCDQPRVLDL
GARKAKKLERVSTDVMDDVLAKLITLIS

Download         Length: 327 bp

>T80697 NZ_CP023276:c317573-317247 [Polynucleobacter difficilis]
ATGAATCGTGGTGATATTTATTTAGTCTCACTAGATCCAACAGCAGGGCATGAGCAAAAAGGTATGCGCCCGGTATTGGT
AGTTTCACCTGATGCGTTTAATAAGTTGACAAAGGCACCAATCGTAGTGCCCATAACATCTGGTGGGAGTTTTGCAAGAT
TAAGTGGATTTACGGTTGATTTAATTGGCTCTAAAACAATGGGGGTTGTACGATGCGATCAACCAAGAGTACTTGACTTA
GGTGCAAGAAAAGCAAAAAAGTTGGAGCGTGTTTCAACCGATGTAATGGATGATGTATTAGCAAAGCTAATTACACTGAT
TTCATAG

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9209.08 Da        Isoelectric Point: 9.7111

>AT80697 WP_108468472.1 NZ_CP023276:c317827-317573 [Polynucleobacter difficilis]
MQTNLRKVGGSVMLAVSPAFLEELKICPGSIVDVALREGQLIVKPITKPKYTLAELLAKCDPKAKMQKEDKLWLSSSLVG
KEIL

Download         Length: 255 bp

>AT80697 NZ_CP023276:c317827-317573 [Polynucleobacter difficilis]
ATGCAAACAAATTTAAGAAAAGTTGGTGGTTCTGTAATGCTGGCCGTATCCCCTGCATTTTTAGAGGAGCTTAAAATTTG
TCCAGGATCAATCGTGGATGTAGCTCTGCGCGAAGGACAGCTAATAGTAAAACCCATCACTAAACCAAAATATACCTTAG
CAGAGTTATTAGCAAAATGTGATCCCAAAGCCAAAATGCAAAAAGAAGACAAGTTATGGCTTAGCTCGAGCCTTGTTGGC
AAAGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References