Detailed information of TA system TA080696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 307310..307919
• Replicon: chromosome
• Accession: NZ_CP023276
• Organism: Polynucleobacter difficilis strain AM-8B5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AOC34_RS01590
• Protein ID: WP_108468459.1
• Coordinates: 307310..307489 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOC34_RS01595
• Protein ID: WP_108468460.1
• Coordinates: 307500..307919 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOC34_RS01550	302349..303236	+	888	WP_108468451.1	undecaprenyl-diphosphate phosphatase	-
AOC34_RS01555	303244..303450	-	207	WP_108468452.1	putative addiction module antidote protein	-
AOC34_RS01560	303608..303880	-	273	WP_108468453.1	type II toxin-antitoxin system YafQ family toxin	-
AOC34_RS01565	303880..304122	-	243	WP_108468454.1	addiction module antitoxin	-
AOC34_RS01570	304471..304767	+	297	WP_108468455.1	helix-turn-helix transcriptional regulator	-
AOC34_RS01575	304764..305219	+	456	WP_108468456.1	DUF2721 domain-containing protein	-
AOC34_RS01580	305308..306285	+	978	WP_108468457.1	SDR family oxidoreductase	-
AOC34_RS01585	306289..307179	+	891	WP_199908334.1	tRNA (guanosine(46)-N7)-methyltransferase TrmB	-
AOC34_RS01590	307310..307489	+	180	WP_108468459.1	type II toxin-antitoxin system HicA family toxin	Toxin
AOC34_RS01595	307500..307919	+	420	WP_108468460.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AOC34_RS01600	308322..309794	-	1473	WP_108468461.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
AOC34_RS01605	310255..311085	+	831	WP_108468462.1	lytic transglycosylase domain-containing protein	-
AOC34_RS01610	311117..311620	-	504	WP_108468463.1	transcription antitermination factor NusB	-
AOC34_RS01615	311610..312122	-	513	WP_108469990.1	6,7-dimethyl-8-ribityllumazine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6463.67 Da
• Isoelectric Point: 11.0929

>T80696 WP_108468459.1 NZ_CP023276:307310-307489 [Polynucleobacter difficilis]
MNSKQMKKWLELQGATFLPGKGSHLKVFLNGKQSTLPMHGTSELGKGLEAAIKRQLGLK

Nucleotide

• Length: 180 bp

>T80696 NZ_CP023276:307310-307489 [Polynucleobacter difficilis]
ATGAATTCGAAGCAGATGAAAAAGTGGCTAGAGCTACAAGGAGCTACTTTTTTACCTGGTAAAGGTTCTCATTTGAAGGT
ATTTTTAAATGGTAAACAATCTACTTTACCGATGCATGGAACAAGCGAATTGGGTAAAGGTTTAGAGGCTGCAATTAAGC
GGCAACTCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15491.89 Da
• Isoelectric Point: 8.0508

>AT80696 WP_108468460.1 NZ_CP023276:307500-307919 [Polynucleobacter difficilis]
MFNYPVTLTKDGDTILVRFRDIPEAITFGADKEEALLNAVDALETGLSFYVDSRKALPLPSKVRRGERTVSPTALECAKL
GVYQAMTEQGIKKSELARRLGWHMPQVDRLFDLRHASKLDQIEAAALALGRHLEVRIAA

Nucleotide

• Length: 420 bp

>AT80696 NZ_CP023276:307500-307919 [Polynucleobacter difficilis]
ATGTTTAACTATCCGGTAACACTGACAAAAGATGGTGACACTATCTTGGTACGCTTTCGGGATATACCAGAAGCAATTAC
GTTTGGCGCCGATAAAGAAGAGGCATTACTAAATGCAGTAGATGCTTTAGAAACAGGATTATCTTTTTATGTAGATTCTC
GCAAGGCATTGCCATTGCCGAGCAAAGTAAGGCGGGGAGAGCGAACAGTATCCCCGACCGCTCTTGAATGTGCCAAATTA
GGCGTTTATCAGGCAATGACAGAGCAGGGTATTAAGAAGTCGGAATTAGCCAGACGTCTGGGTTGGCATATGCCGCAAGT
CGATCGCTTATTTGATTTGCGTCACGCATCTAAGCTTGATCAAATTGAGGCGGCGGCATTGGCACTTGGACGTCATCTTG
AGGTTCGGATTGCTGCTTAA