Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1411175..1412091 Replicon chromosome
Accession NZ_CP023257
Organism Bacillus subtilis strain TLO3

Toxin (Protein)


Gene name spoIISA Uniprot ID -
Locus tag CLD04_RS07605 Protein ID WP_089172519.1
Coordinates 1411345..1412091 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag CLD04_RS07600 Protein ID WP_003232646.1
Coordinates 1411175..1411345 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLD04_RS07570 1408038..1408364 + 327 WP_095843651.1 XkdW family protein -
CLD04_RS07575 1408361..1408525 + 165 WP_015252267.1 XkdX family protein -
CLD04_RS07580 1408572..1409411 + 840 WP_041351214.1 phage-like element PBSX protein XepA -
CLD04_RS07585 1409464..1409733 + 270 WP_041351215.1 hemolysin XhlA family protein -
CLD04_RS07590 1409746..1410009 + 264 WP_041351216.1 phage holin -
CLD04_RS07595 1410022..1410915 + 894 WP_095843652.1 N-acetylmuramoyl-L-alanine amidase -
CLD04_RS21565 1410952..1411089 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
CLD04_RS07600 1411175..1411345 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
CLD04_RS07605 1411345..1412091 - 747 WP_089172519.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
CLD04_RS07610 1412201..1413202 - 1002 WP_014479569.1 inorganic phosphate transporter -
CLD04_RS07615 1413215..1413832 - 618 WP_003218470.1 DUF47 domain-containing protein -
CLD04_RS07620 1414108..1415424 - 1317 WP_041351218.1 serine/threonine exchanger -
CLD04_RS07625 1415813..1416763 + 951 WP_014476561.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1376915..1454375 77460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29121.60 Da        Isoelectric Point: 4.6191

>T80676 WP_089172519.1 NZ_CP023257:c1412091-1411345 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMFAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T80676 NZ_CP023257:c1412091-1411345 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTTCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT80676 WP_003232646.1 NZ_CP023257:c1411345-1411175 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT80676 NZ_CP023257:c1411345-1411175 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References