Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 564283..564919 Replicon chromosome
Accession NZ_CP023257
Organism Bacillus subtilis strain TLO3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CLD04_RS03005 Protein ID WP_003156187.1
Coordinates 564569..564919 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag CLD04_RS03000 Protein ID WP_003225183.1
Coordinates 564283..564564 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLD04_RS02980 560643..561242 - 600 WP_041351790.1 rhomboid family intramembrane serine protease -
CLD04_RS02985 561337..561702 + 366 WP_014475874.1 holo-ACP synthase -
CLD04_RS02990 561868..562884 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
CLD04_RS02995 562998..564167 + 1170 WP_017697002.1 alanine racemase -
CLD04_RS03000 564283..564564 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CLD04_RS03005 564569..564919 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CLD04_RS03010 565034..565858 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
CLD04_RS03015 565863..566228 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
CLD04_RS03020 566232..566633 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
CLD04_RS03025 566645..567652 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
CLD04_RS03030 567714..568043 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
CLD04_RS03035 568040..568522 + 483 WP_003234299.1 anti-sigma B factor RsbW -
CLD04_RS03040 568488..569276 + 789 WP_095843485.1 RNA polymerase sigma factor SigB -
CLD04_RS03045 569276..569875 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T80675 WP_003156187.1 NZ_CP023257:564569-564919 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T80675 NZ_CP023257:564569-564919 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT80675 WP_003225183.1 NZ_CP023257:564283-564564 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT80675 NZ_CP023257:564283-564564 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References