Detailed information of TA system TA080673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 2947084..2947594
• Replicon: chromosome
• Accession: NZ_CP023256
• Organism: Alcaligenes faecalis strain c16

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4P8JRN6
• Locus tag: CLH39_RS13520
• Protein ID: WP_060186046.1
• Coordinates: 2947331..2947594 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A4P8JMX7
• Locus tag: CLH39_RS13515
• Protein ID: WP_060186045.1
• Coordinates: 2947084..2947338 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLH39_RS13470	2942313..2942732	-	420	WP_203144898.1	hypothetical protein	-
CLH39_RS13475	2942907..2943155	-	249	WP_137430852.1	hypothetical protein	-
CLH39_RS13480	2943152..2943532	-	381	WP_203144899.1	hypothetical protein	-
CLH39_RS13485	2943546..2943869	-	324	WP_203144900.1	hypothetical protein	-
CLH39_RS13490	2943877..2944584	-	708	WP_137430850.1	hypothetical protein	-
CLH39_RS13495	2944568..2945365	-	798	WP_203144901.1	hypothetical protein	-
CLH39_RS13500	2945469..2945948	+	480	WP_203144902.1	hypothetical protein	-
CLH39_RS13505	2945902..2946591	+	690	WP_088453217.1	LexA family transcriptional regulator	-
CLH39_RS13510	2946628..2946894	-	267	WP_035275328.1	hypothetical protein	-
CLH39_RS13515	2947084..2947338	+	255	WP_060186045.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CLH39_RS13520	2947331..2947594	+	264	WP_060186046.1	Txe/YoeB family addiction module toxin	Toxin
CLH39_RS13530	2948604..2949089	-	486	WP_060186047.1	DUF4440 domain-containing protein	-
CLH39_RS13535	2949368..2950126	+	759	WP_009459460.1	M48 family metallopeptidase	-
CLH39_RS13540	2950465..2950866	+	402	WP_026484692.1	hypothetical protein	-
CLH39_RS13545	2950863..2951333	+	471	WP_115635061.1	GNAT family N-acetyltransferase	-
CLH39_RS13550	2951371..2951889	+	519	WP_009459463.1	DedA family protein	-
CLH39_RS13555	2951896..2952240	+	345	WP_009459464.1	quaternary ammonium compound resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10354.86 Da
• Isoelectric Point: 9.0258

>T80673 WP_060186046.1 NZ_CP023256:2947331-2947594 [Alcaligenes faecalis]
MTDRLLCWTDAAWIDYVYWQGQDRKTLKRINKLIEEVRRAPFDGIGKPEALKENLSGFWSRRIDDTNRLVYAVDKASITI
ISCRYHY

Nucleotide

• Length: 264 bp

>T80673 NZ_CP023256:2947331-2947594 [Alcaligenes faecalis]
ATGACTGACCGTTTGTTATGCTGGACTGACGCTGCCTGGATTGACTATGTGTATTGGCAAGGCCAGGACCGCAAGACATT
GAAGCGTATCAACAAACTGATAGAAGAAGTACGACGTGCGCCATTCGATGGCATAGGCAAACCTGAAGCGCTTAAAGAAA
ATCTGTCCGGCTTTTGGTCAAGACGCATTGATGACACGAATCGCCTGGTCTATGCCGTAGACAAGGCAAGCATCACCATC
ATTTCTTGTCGTTATCATTATTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9370.53 Da
• Isoelectric Point: 4.7778

>AT80673 WP_060186045.1 NZ_CP023256:2947084-2947338 [Alcaligenes faecalis]
MKIISFSEARNSLKAVLDGVVDDASTTIITRRDAEDAVVMSLDYYNSLMETVHLLRSPANAEHLSRSIEQYRAGKAMPRE
PSDD

Nucleotide

• Length: 255 bp

>AT80673 NZ_CP023256:2947084-2947338 [Alcaligenes faecalis]
ATGAAAATCATCTCGTTTTCAGAAGCGCGCAACAGCTTGAAAGCTGTACTGGATGGCGTAGTCGATGACGCCAGCACCAC
CATCATCACGCGTCGTGATGCGGAAGACGCTGTAGTCATGTCACTCGATTACTACAACAGCTTGATGGAAACCGTCCATC
TGCTGCGCTCCCCCGCTAATGCGGAACACCTGAGCCGCTCTATCGAACAATATCGTGCTGGCAAAGCCATGCCACGCGAG
CCGAGCGATGACTGA

Structures

Toxin

• AlphaFold DB: A0A4P8JRN6

Antitoxin

• AlphaFold DB: A0A4P8JMX7