Detailed information of TA system TA080669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 4931801..4932306
• Replicon: chromosome
• Accession: NZ_CP023255
• Organism: Pseudomonas aeruginosa strain CCUG 70744

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: CLH63_RS23800
• Protein ID: WP_003083773.1
• Coordinates: 4931801..4932082 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: CLH63_RS23805
• Protein ID: WP_003083775.1
• Coordinates: 4932079..4932306 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLH63_RS23775	4927052..4928401	+	1350	WP_003119513.1	C4-dicarboxylate transporter DctA	-
CLH63_RS23780	4928450..4929136	+	687	WP_003083762.1	FadR family transcriptional regulator	-
CLH63_RS23785	4929237..4929971	+	735	WP_003117208.1	GntR family transcriptional regulator	-
CLH63_RS23790	4930175..4930561	+	387	WP_003083767.1	aegerolysin family protein	-
CLH63_RS23795	4930593..4931501	-	909	WP_003083769.1	LysR family transcriptional regulator	-
CLH63_RS23800	4931801..4932082	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
CLH63_RS23805	4932079..4932306	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
CLH63_RS23810	4932482..4933102	-	621	WP_031298866.1	hypothetical protein	-
CLH63_RS23815	4933203..4933703	+	501	WP_003101228.1	LEA type 2 family protein	-
CLH63_RS23820	4933776..4934117	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
CLH63_RS23825	4934199..4935626	-	1428	WP_023464187.1	GABA permease	-
CLH63_RS23830	4935795..4937288	-	1494	WP_016263977.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T80669 WP_003083773.1 NZ_CP023255:c4932082-4931801 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T80669 NZ_CP023255:c4932082-4931801 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT80669 WP_003083775.1 NZ_CP023255:c4932306-4932079 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT80669 NZ_CP023255:c4932306-4932079 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9