Detailed information of TA system TA080667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2789206..2789792
• Replicon: chromosome
• Accession: NZ_CP023255
• Organism: Pseudomonas aeruginosa strain CCUG 70744

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: CLH63_RS13525
• Protein ID: WP_003120987.1
• Coordinates: 2789493..2789792 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CLH63_RS13520
• Protein ID: WP_003448662.1
• Coordinates: 2789206..2789496 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLH63_RS13500	2784357..2784566	+	210	WP_003105733.1	cold-shock protein	-
CLH63_RS13505	2784788..2786677	+	1890	WP_016851610.1	hypothetical protein	-
CLH63_RS13510	2786674..2788650	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
CLH63_RS13515	2788791..2789135	+	345	WP_016851612.1	hypothetical protein	-
CLH63_RS13520	2789206..2789496	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
CLH63_RS13525	2789493..2789792	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLH63_RS13530	2789994..2791118	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
CLH63_RS13535	2791118..2792827	+	1710	WP_110183959.1	PilN family type IVB pilus formation outer membrane protein	-
CLH63_RS13540	2792831..2794156	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
CLH63_RS13545	2794146..2794679	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2762227..2856704	94477

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T80667 WP_003120987.1 NZ_CP023255:c2789792-2789493 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T80667 NZ_CP023255:c2789792-2789493 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT80667 WP_003448662.1 NZ_CP023255:c2789496-2789206 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT80667 NZ_CP023255:c2789496-2789206 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73