Detailed information of TA system TA080666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 2714227..2715485
• Replicon: chromosome
• Accession: NZ_CP023255
• Organism: Pseudomonas aeruginosa strain CCUG 70744

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A241XTS2
• Locus tag: CLH63_RS13115
• Protein ID: WP_023464370.1
• Coordinates: 2714227..2714982 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A241XST8
• Locus tag: CLH63_RS13120
• Protein ID: WP_023464369.1
• Coordinates: 2715117..2715485 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLH63_RS13105	2709398..2711941	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
CLH63_RS13110	2712096..2713457	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
CLH63_RS13115	2714227..2714982	-	756	WP_023464370.1	RES family NAD+ phosphorylase	Toxin
CLH63_RS13120	2715117..2715485	-	369	WP_023464369.1	DUF2384 domain-containing protein	Antitoxin
CLH63_RS13125	2715558..2716844	-	1287	WP_023464368.1	FAD-binding oxidoreductase	-
CLH63_RS13130	2716900..2718489	-	1590	WP_023464367.1	aldehyde dehydrogenase family protein	-
CLH63_RS13135	2718567..2719043	+	477	WP_019725934.1	hypothetical protein	-
CLH63_RS33410	2719046..2719321	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28226.37 Da
• Isoelectric Point: 7.6839

>T80666 WP_023464370.1 NZ_CP023255:c2714982-2714227 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAECAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRASQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGAPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T80666 NZ_CP023255:c2714982-2714227 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTACGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAATGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTTCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGGCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13395.68 Da
• Isoelectric Point: 9.9874

>AT80666 WP_023464369.1 NZ_CP023255:c2715485-2715117 [Pseudomonas aeruginosa]
MSVLIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT80666 NZ_CP023255:c2715485-2715117 [Pseudomonas aeruginosa]
ATGAGCGTTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A241XTS2

Antitoxin

• AlphaFold DB: A0A241XST8