Detailed information of TA system TA080658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3634634..3636315
• Replicon: chromosome
• Accession: NZ_CP023249
• Organism: Klebsiella pneumoniae strain CCUG 70742

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A6TH06
• Locus tag: CLH64_RS18125
• Protein ID: WP_015959269.1
• Coordinates: 3634634..3635509 (-)
• Length: 292 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: CLH64_RS18130
• Protein ID: WP_004177809.1
• Coordinates: 3635557..3636315 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLH64_RS18105	3629940..3631460	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
CLH64_RS18110	3631457..3632455	+	999	WP_002885172.1	ribose ABC transporter permease	-
CLH64_RS18115	3632486..3633427	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
CLH64_RS18120	3633502..3634509	+	1008	WP_015959270.1	carbohydrate kinase family protein	-
CLH64_RS18125	3634634..3635509	-	876	WP_015959269.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CLH64_RS18130	3635557..3636315	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
CLH64_RS18135	3636401..3637129	-	729	WP_032413197.1	response regulator transcription factor	-
CLH64_RS18140	3637402..3639549	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
CLH64_RS28335	3639546..3639689	-	144	WP_163578345.1	hypothetical protein	-
CLH64_RS18145	3640011..3640697	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (7-264)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 292 a.a.
• Molecular weight: 32990.17 Da
• Isoelectric Point: 5.4637

>T80658 WP_015959269.1 NZ_CP023249:c3635509-3634634 [Klebsiella pneumoniae]
MQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQAGMSAVLQANRSDE
MRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQHPRDFYDVKLLLDAQ
ELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKSCITQQDVDFLLSFK
RGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 876 bp

>T80658 NZ_CP023249:c3635509-3634634 [Klebsiella pneumoniae]
ATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATTTGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGA
TTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAG
ATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAAGATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACC
ACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAG
GAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTATTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCC
TCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCCTGTATCACACAACAGGATGTCGATTTTTTACTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT80658 WP_004177809.1 NZ_CP023249:c3636315-3635557 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT80658 NZ_CP023249:c3636315-3635557 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A6TH06

Antitoxin

• AlphaFold DB: A6TH05