Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2737066..2737685 Replicon chromosome
Accession NZ_CP023249
Organism Klebsiella pneumoniae strain CCUG 70742

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CLH64_RS13730 Protein ID WP_002892050.1
Coordinates 2737467..2737685 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CLH64_RS13725 Protein ID WP_002892066.1
Coordinates 2737066..2737440 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLH64_RS13715 2732218..2733411 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CLH64_RS13720 2733434..2736580 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CLH64_RS13725 2737066..2737440 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CLH64_RS13730 2737467..2737685 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CLH64_RS13735 2737848..2738414 + 567 WP_002892030.1 maltose O-acetyltransferase -
CLH64_RS27825 2738386..2738526 - 141 WP_004147370.1 hypothetical protein -
CLH64_RS13740 2738547..2739017 + 471 WP_020802585.1 YlaC family protein -
CLH64_RS13745 2738992..2740443 - 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
CLH64_RS13750 2740544..2741242 + 699 WP_023287311.1 GNAT family N-acetyltransferase -
CLH64_RS13755 2741239..2741379 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CLH64_RS13760 2741379..2741642 - 264 WP_032432663.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T80655 WP_002892050.1 NZ_CP023249:2737467-2737685 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T80655 NZ_CP023249:2737467-2737685 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT80655 WP_002892066.1 NZ_CP023249:2737066-2737440 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT80655 NZ_CP023249:2737066-2737440 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References